Department of Chemistry, State University College of Forestry, Syracuse, New York.
Plant Physiol. 1966 Feb;41(2):298-302. doi: 10.1104/pp.41.2.298.
Growth of the excised axis of Phaseolus vulgaris L. (var. White Marrowfat) begins after a 7-hour incubation in buffer or water at 26 degrees . Growth, as measured by axis elongation or fresh weight increase, is linear for at least 8 hours with a resultant fresh weight increase of approximately 65%. Cell elongation begins 4 or 5 hours prior to cell division and 5 or 6 hours prior to radicle protrusion in the intact seed.The initiation of axis elongation is apparently dependent on synthesis of RNA and protein. Both actinomycin D and puromycin inhibit the initiation of elongation. Actinomycin I) inhibits the incorporation of ATP-8-C(14) into axis RNA and C(14)-leucine into protein, while puromycin inhibits the incorporation of C(14)-leucine into axis protein.The respiratory rate of the axes increases sharply at about the time of initiation of cell elongation. Dinitrophenol initially increases O(2) uptake by the axes, but at the end of 15 hours the rates of O(2) uptake by control or dinitrophenol-treated axes are approximately the same.
菜豆（L. var. White Marrowfat）切段在缓冲液或水中于 26℃孵育 7 小时后开始生长。至少在 8 小时内，通过轴伸长或鲜重增加来测量生长，其鲜重增加约 65%。细胞伸长在细胞分裂前 4 或 5 小时和在完整种子中胚根突出前 5 或 6 小时开始。轴伸长的开始显然依赖于 RNA 和蛋白质的合成。放线菌素 D 和嘌呤霉素均抑制伸长的开始。放线菌素 D 抑制 ATP-8-C(14)掺入轴 RNA 和 C(14)-亮氨酸掺入蛋白质，而嘌呤霉素抑制 C(14)-亮氨酸掺入轴蛋白质。在细胞伸长开始时，轴的呼吸速率急剧增加。二硝基苯酚最初增加轴对 O(2)的摄取，但在 15 小时结束时，对照或二硝基苯酚处理的轴对 O(2)的摄取速率大致相同。
