Sprey B
Institut für Botanik und Mikrobiologie der Kernforschungsanlage Jülich, Jülich, Deutschland.
Planta. 1967 Jun;78(2):115-33. doi: 10.1007/BF00406645.
By means of serial sections of etioplasts of Hordeum vulgare the spatial arrangement of nucleoplasma-like regions before and during plastid division was investigated. 1. After aldehyde-osmium fixation the etioplasts exhibit areas of low electrondensity containing DNA-fibers 50-100 Å thick which are preferentially stained by uranyl-acetate. 2. As could be shown by a series of thin sections, etioplasts 2-3 μ long predominantly contain only one polymorphous nucleoplasma-like region which may be split in such a fashion as to show more than one of these low electron density areas in a single section. 3. Two DNA-containing nucleoplasma-like regions could be demonstrated in enlarged plastids (5-6 μ in length). It is tentatively concluded that the original regions are divided before plastid division. 4. In some cases the DNA-containing area showed a parallel arrangement to the constriction of the dividing plastid. In these cases it is assumed that the DNA-containing area is distributed between the daughter plastids during plastid division.
通过对大麦黄化质体的连续切片，研究了质体分裂前和分裂过程中类核质区域的空间排列。1. 经醛锇固定后，黄化质体呈现出低电子密度区域，其中含有50 - 100 Å厚的DNA纤维，这些纤维优先被醋酸铀染色。2. 一系列薄片显示，2 - 3微米长的黄化质体主要仅含有一个多形态的类核质区域，该区域可能以在单个切片中显示不止一个低电子密度区域的方式分裂。3. 在较大的质体（长度为5 - 6微米）中可以证明有两个含DNA的类核质区域。初步推断，原始区域在质体分裂前就已分开。4. 在某些情况下，含DNA区域与分裂质体的缢缩处呈平行排列。在这些情况下，假定含DNA区域在质体分裂期间分布于子质体之间。
