Specht-Jürgensen I, Bachofen R
Institut für Allgemeine Botanik der Universität Zürich, Zürich, Schweiz.
Planta. 1968 Sep;79(3):222-34. doi: 10.1007/BF00396029.
Light induced the formation of an energy pool in chloroplast fragments which was emptied within 20-30 min in the dark, while an ATP-Pi exchange was going on.The exchange reaction was dependent on Mg(2+) and was inhibited by NH 4 (+) but not by ADP. The optimum pH in the light and in the dark stage of the reaction lay between pH 7.5 and 8.The high energy intermediate was formed only in the presence of DTT in the light stage; phosphate had no influence on the formation of the intermediate in that period, but increased its stability in the dark.The relation between both high energy intermediates, that of the light-induced ATP-Pi exchange reaction and that of the light-induced ATP synthesis, is discussed in its meaning for the hypothesis of chemiosmotic phosphorylation.
光诱导叶绿体片段中形成一个能量池，在黑暗中，该能量池会在20 - 30分钟内排空，同时会发生ATP - Pi交换。该交换反应依赖于Mg(2+)，并受到NH 4(+)的抑制，但不受ADP的抑制。反应的光照阶段和黑暗阶段的最适pH值在7.5至8之间。高能中间体仅在光照阶段存在二硫苏糖醇（DTT）时形成；在此期间，磷酸盐对中间体的形成没有影响，但在黑暗中会增加其稳定性。文中讨论了光诱导的ATP - Pi交换反应的高能中间体与光诱导的ATP合成的高能中间体之间的关系，及其对化学渗透磷酸化假说的意义。
