Moshiri Mohammad, Vahabzadeh Maryam, Etemad Leila, Hosseinzadeh Hossein
Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran J Pharm Res. 2013 Fall;12(4):897-902.
Diazinon (DZN) is a synthetic organophosphorus (OPs) insecticide widely used in agricultural and household applications. OPs, particularly DZN, are highly lipid soluble liquids. Intravenous lipid emulsion (ILE) has been shown to reduce toxicity caused by some lipid soluble agents. We evaluated the antidote effect of ILE on acute toxicity of DZN. Twenty-four Sprague-Dawley female rats weighting 200-250 g were treated orally with dose of 480 mg/ kg of DZN gavaged at the volume of 0.5 mL/kg. Thirty minutes after administration of DZN, two groups were treated by either ILE 10% (ILE10) or normal saline (NS) (16 mL/kg) (NS16) that were infused for the duration of 15 minutes. Another two groups were also treated by either ILE 20% (ILE20) or NS (10 mL/kg: NS10) as above. The changes in body weight, diarrhea score, muscular power, fasciculation, convulsions and mortality rate of the animals were all monitored immediately after infusions and then every 6 h up to 48 h. There was no significant difference in animals mean weight between different groups during the observation period. In addition, during the 48-hour observation we could not find any difference in muscular power and diarrhea score between groups of ILE20-NS10 and ILE10-NS16 in comparison with each other, and neither ILE 10% nor ILE %20 could not reduce mortality rate of animals or increase the survival time of rats. In conclusion, ILE seems to be unable to reverse DZN acute toxicity and it might be due to conversion of DZN to potent and less lipid soluble agent.
二嗪农（DZN）是一种合成有机磷（OPs）杀虫剂，广泛用于农业和家庭应用。OPs，特别是DZN，是高度脂溶性液体。静脉注射脂质乳剂（ILE）已被证明可降低某些脂溶性药物引起的毒性。我们评估了ILE对DZN急性毒性的解毒作用。将24只体重200 - 250 g的Sprague-Dawley雌性大鼠按480 mg/kg的剂量口服给予DZN，灌胃体积为0.5 mL/kg。给予DZN 30分钟后，两组分别接受10%的ILE（ILE10）或生理盐水（NS）（16 mL/kg）（NS16）治疗，持续输注15分钟。另外两组也分别接受20%的ILE（ILE20）或上述NS（1 mL/kg：NS10）治疗。在输注后立即监测动物的体重、腹泻评分、肌肉力量、肌束震颤、惊厥和死亡率变化，然后每6小时监测一次，直至48小时。在观察期内，不同组动物的平均体重无显著差异。此外，在48小时观察期内，我们发现ILE20 - NS10组和ILE10 - NS16组之间在肌肉力量和腹泻评分方面相互比较没有差异，10%的ILE和20%的ILE均不能降低动物死亡率或增加大鼠存活时间。总之，ILE似乎无法逆转DZN的急性毒性，这可能是由于DZN转化为强效且脂溶性较低的药物所致。
