Wu Wei, Zheng Limin, Chen Hongyan, Jia Dongsheng, Li Fan, Wei Taiyun
Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, PR China ; Key Laboratory of Agricultural Biodiversity for Pest Management of China's Ministry of Education, Yunnan Agricultural University, Kunming, Yunnan, PR China.
Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, PR China.
PLoS One. 2014 Feb 11;9(2):e88636. doi: 10.1371/journal.pone.0088636. eCollection 2014.
Rice stripe virus (RSV), a tenuivirus, is transmitted by small brown planthopper (SBPH) in a persistent-propagative manner. In this study, sequential infection of RSV in the internal organs of SBPH after ingestion of virus indicated that RSV initially infected the midgut epithelium, and then progressed to the visceral muscle tissues, through which RSV spread to the entire alimentary canal. Finally, RSV spread into the salivary glands and reproductive system. During viral infection, the nonstructural protein NS4 of RSV formed cytoplasmic inclusions in various tissues of viruliferous SBPH. We demonstrated that the ribonucleoprotein particles of RSV were closely associated with NS4-specific inclusions in the body of viruliferous SBPH through a direct interaction between NS4 and nucleoprotein of RSV. Moreover, the knockdown of NS4 expression due to RNA interference induced by dsRNA from NS4 gene significantly prevented the spread of RSV in the bodies of SBPHs without a significant effect on viral replication in continuous cell culture derived from SBPH. All these results suggest that the nonstructural protein NS4 of RSV plays a critical role in viral spread by the vector insects.
水稻条纹病毒（RSV）是一种纤细病毒，由灰飞虱以持久增殖的方式传播。在本研究中，灰飞虱摄入病毒后RSV在其内部器官中的连续感染表明，RSV最初感染中肠上皮，然后进展到内脏肌肉组织，RSV通过该组织扩散到整个消化道。最后，RSV扩散到唾液腺和生殖系统。在病毒感染期间，RSV的非结构蛋白NS4在带毒灰飞虱的各种组织中形成细胞质内含物。我们证明，通过NS4与RSV核蛋白之间的直接相互作用，RSV的核糖核蛋白颗粒与带毒灰飞虱体内的NS4特异性内含物密切相关。此外，由NS4基因的dsRNA诱导的RNA干扰导致NS4表达的敲低显著阻止了RSV在灰飞虱体内的传播，而对来自灰飞虱的连续细胞培养物中的病毒复制没有显著影响。所有这些结果表明，RSV的非结构蛋白NS4在病毒通过媒介昆虫传播中起关键作用。
