Li Bo-Xue, Qi Yu-Hua, Zhang Chuan-Xi, Chen Jian-Ping, Li Jun-Min, Lu Gang
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
Insects. 2024 Feb 22;15(3):149. doi: 10.3390/insects15030149.
Many hosts utilize the ubiquitin system to defend against viral infection. As a key subunit of the ubiquitin system, the role of polyubiquitin in the viral infection of insects is unclear. Here, we identified the full-length cDNA of the polyubiquitin-C (UBC) gene in , the small brown planthopper (SBPH). was expressed in various tissues and was highly expressed in salivary glands, midgut, and reproductive systems. Furthermore, the expression profiles in the developmental stages showed that was ubiquitously expressed in seven developmental stages and was highest expressed in female adults with SBPH. qRT-PCR analyses indicated that rice stripe virus (RSV) infection promoted the expression. Knockdown of mRNA via RNA interference increased RSV accumulation. These findings suggest that LsUBC inhibits RSV accumulation in .
许多宿主利用泛素系统来抵御病毒感染。作为泛素系统的关键亚基，多聚泛素在昆虫病毒感染中的作用尚不清楚。在此，我们鉴定了灰飞虱中多聚泛素-C（UBC）基因的全长cDNA。该基因在多种组织中表达，在唾液腺、中肠和生殖系统中高表达。此外，其在发育阶段的表达谱表明，该基因在七个发育阶段均有广泛表达，在灰飞虱雌成虫中表达量最高。qRT-PCR分析表明，水稻条纹病毒（RSV）感染促进了该基因的表达。通过RNA干扰敲低该基因的mRNA会增加RSV的积累。这些发现表明，LsUBC抑制了RSV在灰飞虱中的积累。
