Breborowicz J
Department of Pathomorphology, Karol Marcinkowski Academy of Medicine, Poznan, Poland.
Tumour Biol. 1988;9(1):3-14. doi: 10.1159/000217540.
The data available so far on the microheterogeneity of human AFP are reviewed. Molecular and developmental bases for the microheterogeneity of human AFP are discussed. Special attention is paid to the application of the studies on AFP microheterogeneity for the prenatal diagnosis of developmental malformations and for the differential diagnosis of some cancers. The results obtained using different methods are compared with special reference to lectin affinity electrophoresis and lectin affinity chromatography. In addition, a self-explanatory nomenclature of AFP variants is proposed in order to simplify the comparison of results obtained by different investigators. This has already been accepted by several investigators. The eventual simplification of methods applicable for clinical purposes and especially the difficulties encountered in experiments with monoclonal antibodies are also discussed.
本文综述了目前有关人甲胎蛋白（AFP）微观异质性的可用数据。讨论了人AFP微观异质性的分子和发育基础。特别关注AFP微观异质性研究在发育畸形产前诊断和某些癌症鉴别诊断中的应用。对使用不同方法获得的结果进行了比较，特别提及凝集素亲和电泳和凝集素亲和色谱法。此外，为简化不同研究者所得结果的比较，提出了一种无需解释的AFP变体命名法，该命名法已被多位研究者接受。还讨论了适用于临床目的的方法最终简化的问题，尤其是单克隆抗体实验中遇到的困难。
