Breborowicz J, Mackiewicz A, Breborowicz D
Scand J Immunol. 1981 Jul;14(1):15-20. doi: 10.1111/j.1365-3083.1981.tb00179.x.
Microheterogeneity of alpha-fetoprotein (AFP) present in the sera of 76 patients was studied by lectin affino-immunoelectrophoresis. Seventeen patients had benign liver disorders and the remaining 59 patients were treated for primary or secondary liver cancer or yolk sac tumour. By means of Con A, AFP was divided into two variants, while lentil lectin (LCA) made it possible to separate AFP in three variants. In some patients the relative concentrations of Con A and LCA AFP variants were similar; these patients were believed to produce AFP of the same 'profile'. Fourteen AFP profiles were observed by estimation of the area enclosed by precipitates corresponding to respective AFP variants. It was also possible to estimate the AFP profile on the basis of a simple visual analysis of the electrophoretic plates. The obtained results indicate that the AFP profiles of patients with cancer were variable. In spite of variations of the AFP profile in cancer patients, in most cases it was possible to differentiate primary liver cancer from yolk sac tumour and from liver metastases of cancer. In addition, in two-thirds of hepatoma patients the AFP profile was different from the profile observed in patients with benign liver disorders.
采用凝集素亲和免疫电泳法研究了76例患者血清中甲胎蛋白（AFP）的微观异质性。17例患者患有良性肝脏疾病，其余59例患者接受了原发性或继发性肝癌或卵黄囊瘤的治疗。借助刀豆球蛋白A（Con A），AFP可分为两种变体，而扁豆凝集素（LCA）则可将AFP分离为三种变体。在一些患者中，Con A和LCA AFP变体的相对浓度相似；这些患者被认为产生具有相同“谱型”的AFP。通过估计与各个AFP变体相对应的沉淀所包围的面积，观察到了14种AFP谱型。基于对电泳板的简单目视分析，也有可能估计AFP谱型。所得结果表明，癌症患者的AFP谱型是可变的。尽管癌症患者的AFP谱型存在差异，但在大多数情况下，仍有可能将原发性肝癌与卵黄囊瘤以及癌症肝转移区分开来。此外，在三分之二的肝癌患者中，AFP谱型与良性肝脏疾病患者所观察到的谱型不同。
