Portnoï D, Lundkvist I, Coutinho A
Laboratory of Immunobiology, Pasteur Institute, Paris, France.
Scand J Immunol. 1988 Apr;27(4):445-50. doi: 10.1111/j.1365-3083.1988.tb02370.x.
Analyses of B-cell repertoires by mitogen-driven limiting dilution systems (LDA) followed by specific antibody detection on immobilized antigens (ELISA), while constituting the only method available to determine absolute frequencies of any given specificity, provide no indications as to the functional ability of clonal precursors scored as positive to actually respond to antigen in vivo. We have now addressed this question by quantitating dextran alpha 1----6-specific clonal precursors among small, resting, and large activated B cells in the spleens of mice, before and after priming with optimal doses of antigen. The results demonstrate that virtually all B cells scored as antigen-specific in LDA/ELISA systems do respond to antigen and undergo blast transformation after priming in vivo.
通过丝裂原驱动的有限稀释系统（LDA）分析B细胞库，随后在固定抗原上进行特异性抗体检测（ELISA），虽然这是确定任何给定特异性绝对频率的唯一可用方法，但对于在体内被判定为对抗原有反应的克隆前体的功能能力却没有提供任何指示。我们现在通过定量小鼠脾脏中在最佳剂量抗原致敏前后的小的、静止的和大的活化B细胞中α1----6特异性葡聚糖克隆前体来解决这个问题。结果表明，在LDA/ELISA系统中被判定为抗原特异性的几乎所有B细胞在体内致敏后确实对抗原产生反应并经历母细胞转化。
