Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, No. 127 Changle West Road, Xi׳an 710032, Shaanxi, PR China.
Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi׳an 710032, PR China.
J Ethnopharmacol. 2014 Mar 28;152(3):553-60. doi: 10.1016/j.jep.2014.02.001. Epub 2014 Feb 11.
As a well-known traditional Chinese medicine the root bark of Aralia taibaiensis has multiple pharmacological activities, including relieving rheumatism, promoting blood circulation to arrest pain, inducing diuresis to reduce edema, and antidiabetic action. It has long been used as a folk medicine for the treatment of traumatic injury, rheumatic arthralgia, nephritis, edema, hepatitis and diabetes mellitus in China.
To evaluate the antihyperglycemic, hypolipidemic and antioxidant activities of total saponins extracted from Aralia taibaiensis (SAT) in experimental type 2 diabetic mellitus (T2DM) rats.
Acute toxicity was studied in rats to determine the safe oral dose of SAT. Then, SAT was given orally to normal and streptozotocin-nicotinamide induced T2DM rats at 80, 160 and 320 mg/kg doses for a series of 28 days to determine the antihyperglycemic activity. Glibenclamide (600 μg/kg), a standard antidiabetic drug, was used as a positive control drug. At the end of treatment, biochemical parameters and antioxidant levels were measured to evaluate the hypolipidemic and antioxidant activities of SAT.
Oral administration of SAT did not exhibit toxicity and death at a dose not more than 2000 mg/kg. SAT dose-dependently improved the symptoms of polydipsia, polyuria, polyphagia and weight loss in diabetic rats. Compared with diabetic control group, administration of 320 mg/kg SAT resulted in significant (P<0.05) fall in the levels of fasting blood glucose, glycosylated hemoglobin, creatinine, urea, alanine transarninase, aspartate aminotransferase, total cholesterol, triglycerides, low density lipoprotein cholesterol and malondialdehyde, but significant (P<0.05) increase in the levels of serum insulin, superoxide dismutase and reduced glutathione. However, SAT did not have any effect on the normal rats.
SAT had excellent antihyperglycemic, hypolipidemic and antioxidant activities in T2DM rats and might be a promising drug in the therapy of diabetes mellitus and its complications.
作为一种著名的中药，刺五加根皮具有多种药理活性，包括祛风除湿、活血止痛、利尿消肿和降血糖作用。在中国，它长期以来一直被用作民间药物，用于治疗创伤、风湿性关节炎、肾炎、水肿、肝炎和糖尿病。
评价刺五加总皂苷（SAT）对实验性 2 型糖尿病（T2DM）大鼠的降血糖、调血脂和抗氧化作用。
在大鼠中进行急性毒性研究，以确定 SAT 的安全口服剂量。然后，将 SAT 以 80、160 和 320mg/kg 剂量口服给予正常和链脲佐菌素-烟酰胺诱导的 T2DM 大鼠，连续 28 天，以确定其降血糖活性。格列本脲（600μg/kg）作为一种标准的降糖药物作为阳性对照药物。在治疗结束时，测量生化参数和抗氧化水平，以评估 SAT 的调血脂和抗氧化活性。
口服 SAT 在剂量不超过 2000mg/kg 时没有表现出毒性和死亡。SAT 剂量依赖性地改善了糖尿病大鼠多饮、多尿、多食和体重减轻的症状。与糖尿病对照组相比，给予 320mg/kg SAT 可显著（P<0.05）降低空腹血糖、糖化血红蛋白、肌酐、尿素、丙氨酸转氨酶、天冬氨酸转氨酶、总胆固醇、甘油三酯、低密度脂蛋白胆固醇和丙二醛水平，但显著（P<0.05）增加血清胰岛素、超氧化物歧化酶和还原型谷胱甘肽水平。然而，SAT 对正常大鼠没有任何影响。
SAT 对 T2DM 大鼠具有良好的降血糖、调血脂和抗氧化作用，可能是治疗糖尿病及其并发症的有前途的药物。
