UFZ - Helmholtz-Centre for Environmental Research, Department of Environmental Microbiology, Permoserstrasse 15, 04318 Leipzig, Germany.
UFZ - Helmholtz-Centre for Environmental Research, Department of Environmental Microbiology, Permoserstrasse 15, 04318 Leipzig, Germany.
Curr Opin Biotechnol. 2014 Jun;27:134-41. doi: 10.1016/j.copbio.2014.01.011. Epub 2014 Feb 11.
Natural microbial systems are highly dynamic due to the short generation times of the comprised organisms and their rapid and distinct reactions to changing environments. Microbial flow cytometry approaches are capable techniques for following such community dynamics in a fast and inexpensive way. Newly developed bioinformatics tools not only enable quantification of single cell dynamics, they also make nearly on-line evaluation of community attributes possible, enable interpretation of community trends, and reveal possible constraints that influence community structure and function. Microbial flow cytometry is poised to make the microbial cytome accessible for ambitious ecosystem studies. Functions of cells within the cytome can be determined either by cell sorting in combination with other omics-approaches of choice or by simple correlation analyses.
由于组成生物的代时较短，以及它们对环境变化的快速而明显的反应，自然微生物系统具有高度动态性。微生物流动细胞术方法是一种能够快速、廉价地跟踪这种群落动态的技术。新开发的生物信息学工具不仅能够定量单个细胞的动态，还能够实现群落属性的近乎在线评估，使解释群落趋势成为可能，并揭示可能影响群落结构和功能的约束因素。微生物流动细胞术有望使微生物细胞组成为雄心勃勃的生态系统研究提供便利。细胞组内的细胞功能可以通过细胞分选与其他选择的组学方法相结合来确定，也可以通过简单的相关分析来确定。
