Gandhi Gopalsamy Rajiv, Vanlalhruaia Pautu, Stalin Antony, Irudayaraj Santiagu Stephen, Ignacimuthu Savarimuthu, Paulraj Michael Gabriel
Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai 600034, India.
Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai 600034, India.
Food Chem Toxicol. 2014 Apr;66:358-65. doi: 10.1016/j.fct.2014.02.001. Epub 2014 Feb 10.
The aim of this study was to evaluate the antidiabetic activity of Cyamopsis tetragonoloba (L.) Taub. (Fabaceae) beans in high-fat diet (HFD) fed-streptozotocin (STZ)-induced type 2 diabetic rats. Dose dependent response of oral treatment of C. tetragonoloba beans' methanol extract (CTme) (200 and 400mg/kg b wt.) was assessed by measuring fasting blood glucose, changes in body weight, plasma insulin, homeostasis model assessment of insulin resistance (HOMA-IR), total cholesterol, triglycerides, oral glucose tolerance, intraperitoneal insulin tolerance, hepatic glycogen, marker enzymes of carbohydrate metabolism in HFD fed-STZ-induced type 2 diabetic rats. Histology and immunohistochemical analysis of pancreatic islets were also performed. High-performance liquid chromatography (HPLC) analysis of CTme showed the presence of polyphenols such as gallic acid and caffeic acid in the concentrations of 2.46% (W/W) and 0.32% (W/W). CTme significantly reverted the altered biochemical parameters to near normal levels in diabetic rats. Furthermore CTme showed the protective effect on the β-cells of pancreatic tissues in diabetic rats. These findings indicate that C. tetragonoloba beans have therapeutic potential in HFD fed-STZ-induced hyperglycemia; therefore this can be used in the management of type 2 diabetes.
本研究旨在评估瓜尔豆（Cyamopsis tetragonoloba (L.) Taub.，豆科）对高脂饮食（HFD）喂养并经链脲佐菌素（STZ）诱导的2型糖尿病大鼠的抗糖尿病活性。通过测量空腹血糖、体重变化、血浆胰岛素、胰岛素抵抗稳态模型评估（HOMA-IR）、总胆固醇、甘油三酯、口服葡萄糖耐量、腹腔内胰岛素耐量、肝糖原以及HFD喂养并经STZ诱导的2型糖尿病大鼠碳水化合物代谢的标记酶，评估了瓜尔豆甲醇提取物（CTme）（200和400mg/kg体重）口服治疗的剂量依赖性反应。还对胰岛进行了组织学和免疫组织化学分析。CTme的高效液相色谱（HPLC）分析表明存在没食子酸和咖啡酸等多酚，其浓度分别为2.46%（W/W）和0.32%（W/W）。CTme显著将糖尿病大鼠中改变的生化参数恢复到接近正常水平。此外，CTme对糖尿病大鼠胰腺组织的β细胞显示出保护作用。这些发现表明，瓜尔豆对HFD喂养并经STZ诱导的高血糖具有治疗潜力；因此可用于2型糖尿病的管理。
