Schmidley J W
Department of Neurology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-5000.
Anat Rec. 1987 Dec;219(4):363-8. doi: 10.1002/ar.1092190406.
Our recent observation that the basement membranes of brain microvessels do not stain with the cationic dye ruthenium red has raised the question of whether the basement membranes of this and other vascular beds functioning as barriers between blood and neural tissues are deficient in the polyanionic macromolecules, such as glycosaminoglycans, which are responsible for the ruthenium red staining of other vascular basement membranes. We therefore attempted to produce staining in the only barrier-type microvascular basement membrane known to contain heparan sulfate. Bovine retinas were fixed by immersion in aldehyde fixatives containing ruthenium red, buffered with either 10 mM or 100 mM sodium cacodylate. We found discrete, electron-dense deposits of ruthenium red in vascular basement membranes, quite similar to those seen in vascular basement membranes of nonneural tissues after exposure to ruthenium red. These deposits were more distinct and more frequent in tissue exposed to ruthenium red-aldehyde solutions buffered with 10 mM cacodylate. They were not seen if ruthenium red was omitted from the fixative. The results demonstrate that anionic macromolecules in basement membranes of barrier-type microvessels can be stained with cationic dyes, and suggest that the failure of brain microvessels to stain with ruthenium red may be the result of a relative or total lack of polyanion in this basement membrane, or of other unique properties.
我们最近观察到脑微血管的基底膜不能被阳离子染料钌红染色，这就提出了一个问题：作为血液与神经组织之间屏障的这种及其他血管床的基底膜，是否缺乏负责其他血管基底膜钌红染色的多阴离子大分子，如糖胺聚糖。因此，我们试图对已知含有硫酸乙酰肝素的唯一屏障型微血管基底膜进行染色。将牛视网膜浸入含有钌红、用10 mM或100 mM二甲胂酸钠缓冲的醛固定剂中进行固定。我们在血管基底膜中发现了离散的、电子致密的钌红沉积物，与非神经组织的血管基底膜在接触钌红后所见的沉积物非常相似。在暴露于用10 mM二甲胂酸钠缓冲的钌红 - 醛溶液的组织中，这些沉积物更明显且更频繁。如果固定剂中省略钌红，则看不到这些沉积物。结果表明，屏障型微血管基底膜中的阴离子大分子可以被阳离子染料染色，并表明脑微血管不能被钌红染色可能是由于该基底膜中相对或完全缺乏多阴离子，或其他独特性质所致。
