Ovtscharoff W
Acta Histochem. 1975;53(1):117-25.
The ultrastructural distribution of ruthenium red staining material is studied in the cerebral cortex of rat. It is established that the material is located equally along the cell surface of the neurons, glial and endothelial cells, and within a number of cell organelles: mitochondria, smooth endoplasmic reticulum, synaptic vesicles and throughout both membranes of the nuclear envelope. Using digestion with hyaluronidase and trypsin, and lipid extraction an attempt is made to determine the participation of some chemical compounds such as acid mucopolysaccharides, glycoproteins, glycolipids and acid polypeptides in the composition of the ruthenium red-positive material. Moreover, the problem of the optimal conditions for the carrying out of ruthenium red staining in the CNS is discussed.
对大鼠大脑皮质中钌红染色物质的超微结构分布进行了研究。已确定该物质均匀分布于神经元、神经胶质细胞和内皮细胞的细胞表面，以及一些细胞器内：线粒体、滑面内质网、突触小泡和核膜的两层膜中。通过用透明质酸酶和胰蛋白酶消化以及脂质提取，试图确定一些化合物如酸性粘多糖、糖蛋白、糖脂和酸性多肽在钌红阳性物质组成中的参与情况。此外，还讨论了在中枢神经系统中进行钌红染色的最佳条件问题。
