School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, People's Republic of China.
Environ Technol. 2013 Sep-Oct;34(17-20):2881-8. doi: 10.1080/09593330.2013.796003.
A laboratory-scale internal circulation (IC) anaerobic reactor fed with brewery wastewater was operated at 35 degrees C + 1 degrees C. The influent was pumped into the bottom of the IC reactor by a pulse pump, whereas the effluent was drawn from the upper outlet and allowed to flow into the effluent tank. The biogas volume was recorded using a gas container connected to a biogas metre. The results indicated that the maximum organic loading rate (OLR) of the IC reactor was 19.5 kg chemical oxygen demand (COD)/m3/day; at which point, the dominant archaeal populations found in the sludge using the polymerase chain reaction with denaturing gradient gel electrophoresis were Methanosaeta species. The COD removal efficiencies of the reactor exceeded 85%, with a maximum specific methane production rate of 210 mL CH4/g volatile suspended solids (VSS)/day and a coenzyme F420 content of 0.16 micromol/g VSS, respectively. The main archaeal species in the sludge samples at different OLRs varied greatly, as compared with the organisms in the inoculated sludge. The dominant archaeal species in the treated sludge at low OLRs were Methanosarcina species, whereas those at high OLRs were Methanosaeta species.
采用实验室规模的内循环（IC）厌氧反应器，以 35 摄氏度 ± 1 摄氏度处理啤酒废水。通过脉冲泵将进水抽入 IC 反应器底部，而出水则从上部出口流出并流入出水槽。通过与沼气表相连的气体容器来记录沼气体积。结果表明，IC 反应器的最大有机负荷（OLR）为 19.5kg 化学需氧量（COD）/m3/天；此时，使用聚合酶链反应和变性梯度凝胶电泳在污泥中发现的主要古菌种群为产甲烷菌属。反应器的 COD 去除效率超过 85%，最大比甲烷生成速率为 210mL CH4/g 挥发性悬浮固体（VSS）/天，辅酶 F420 含量为 0.16 微摩尔/g VSS。与接种污泥中的生物相比，不同 OLR 下污泥样品中的主要古菌种类差异很大。在低 OLR 下处理污泥中的主要古菌种群为产甲烷菌属，而在高 OLR 下则为产甲烷菌属。
