Asmaa Mat Jusoh Siti, Al-Jamal Hamid Ali Nagi, Ang Cheng Yong, Asan Jamaruddin Mat, Seeni Azman, Johan Muhammad Farid
Department of Haematology, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia E-mail :
Asian Pac J Cancer Prev. 2014;15(1):475-81. doi: 10.7314/apjcp.2014.15.1.475.
Pereskia sacharosa is a genus of cacti widely used in folk medicine for cancer-related treatment. Anti-proliferative effects have been studied in recent years against colon, breast, cervical and lung cancer cell lines, with promising results. We here extended study of anti-proliferative effects to a blood malignancy, leukemia.
Two leukemic cell lines, MV4-11 (acute myeloid leukemia) and K562 (chronic myeloid leukemia), were studied. IC50 concentrations were determined and apoptosis and cell cycle regulation were studied by flow cytometric analysis. The expression of apoptosis and cell-cycle related regulatory proteins was assessed by Western blotting.
P sacharosa inhibited growth of MV4-11 and K562 cells in a dose-dependent manner. The mode of cell death was via induction of intrinsic apoptotic pathways and cell cycle arrest. There was profound up-regulation of cytochrome c, caspases, p21 and p53 expression and repression of Akt and Bcl-2 expression in treated cells.
These results suggest that P sacharosa induces leukemic cell death via apoptosis induction and changes in cell cycle checkpoint, thus deserves further study for anti-leukemic potential.
梨果仙人掌属仙人掌，在民间医学中广泛用于癌症相关治疗。近年来，已对其针对结肠、乳腺、宫颈和肺癌细胞系的抗增殖作用进行了研究，结果令人鼓舞。我们在此将抗增殖作用的研究扩展到一种血液恶性肿瘤——白血病。
研究了两种白血病细胞系，MV4-11（急性髓系白血病）和K562（慢性髓系白血病）。测定IC50浓度，并通过流式细胞术分析研究细胞凋亡和细胞周期调控。通过蛋白质印迹法评估凋亡和细胞周期相关调节蛋白的表达。
梨果仙人掌以剂量依赖性方式抑制MV4-11和K562细胞的生长。细胞死亡模式是通过诱导内源性凋亡途径和细胞周期停滞。在处理的细胞中，细胞色素c、半胱天冬酶、p21和p53的表达显著上调，而Akt和Bcl-2的表达受到抑制。
这些结果表明，梨果仙人掌通过诱导凋亡和改变细胞周期检查点来诱导白血病细胞死亡，因此其抗白血病潜力值得进一步研究。
