Wan Y J, Levi B Z, Ozato K
Laboratory of Development and Molecular Immunity, National Institute of Child Health and Human Development, Bethesda, MD 20892.
J Interferon Res. 1988 Feb;8(1):105-12. doi: 10.1089/jir.1988.8.105.
We show that murine interferons (IFNs)-alpha/beta and -gamma induce expression of the protooncogene c-fos in F9 embryonal carcinoma cells, NIH-3T3 cells, and other tissue culture cells. The induction of c-fos mRNA is rapid and transient in that the mRNA appears within 15 min and disappears by 2 h following IFN treatment. The protein synthesis inhibitor cycloheximide (CHX) also stimulates rapid but more stable expression of c-fos mRNA in these cells. Treatment of these cells with a combination of CHX and IFNs results in superinduction of c-fos mRNA. In contrast, IFNs do not affect c-myc mRNA expression in these cells. These results suggest that c-fos gene expression plays a role in signal transduction following binding of IFNs to the receptors.
我们发现，小鼠α/β干扰素和γ干扰素可诱导F9胚胎癌细胞、NIH-3T3细胞及其他组织培养细胞中原癌基因c-fos的表达。c-fos mRNA的诱导迅速且短暂，在干扰素处理后15分钟内出现，2小时后消失。蛋白质合成抑制剂放线菌酮（CHX）也能刺激这些细胞中c-fos mRNA的快速但更稳定的表达。用CHX和干扰素联合处理这些细胞会导致c-fos mRNA的超诱导。相比之下，干扰素不影响这些细胞中c-myc mRNA的表达。这些结果表明，c-fos基因表达在干扰素与受体结合后的信号转导中起作用。
