• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

双链RNA诱导c-Ha-ras基因表达及对干扰素的需求

Induction of c-Ha-ras gene expression by double-stranded RNA and interferon requirement.

作者信息

Maran A, Goldberg I D, Steinberg B M

机构信息

Long Island Jewish Medical Center, New Hyde Park, New York 11042.

出版信息

Mol Cell Biol. 1990 Aug;10(8):4424-6. doi: 10.1128/mcb.10.8.4424-4426.1990.

DOI:10.1128/mcb.10.8.4424-4426.1990
PMID:2196455
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC361008/
Abstract

The addition of double-stranded RNA (dsRNA) to NIH 3T3 cells led to an increase in the RNA levels of c-Ha-ras. The double-stranded configuration was required for the increase in c-Ha-ras mRNA levels, as heat-denatured dsRNA and single-stranded RNA did not have any effect. Nuclear run-on transcription experiments indicated that the increase in c-Ha-ras mRNA levels stimulated by dsRNA was due to transcriptional activation of the gene. The induction of c-Ha-ras gene expression by dsRNA was inhibited by anti-beta interferon antibodies, suggesting that interferon might mediate the induction.

摘要

将双链RNA（dsRNA）添加到NIH 3T3细胞中会导致c-Ha-ras的RNA水平升高。双链结构是c-Ha-ras mRNA水平升高所必需的，因为热变性的dsRNA和单链RNA没有任何作用。细胞核连续转录实验表明，dsRNA刺激引起的c-Ha-ras mRNA水平升高是由于该基因的转录激活。dsRNA对c-Ha-ras基因表达的诱导作用被抗β干扰素抗体抑制，这表明干扰素可能介导了这种诱导作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36ea/361008/3d6c73e8b804/molcellb00044-0580-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36ea/361008/2fd9baa071f5/molcellb00044-0579-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36ea/361008/07232ea9e203/molcellb00044-0579-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36ea/361008/3d6c73e8b804/molcellb00044-0580-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36ea/361008/2fd9baa071f5/molcellb00044-0579-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36ea/361008/07232ea9e203/molcellb00044-0579-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36ea/361008/3d6c73e8b804/molcellb00044-0580-a.jpg

相似文献

1
Induction of c-Ha-ras gene expression by double-stranded RNA and interferon requirement.双链RNA诱导c-Ha-ras基因表达及对干扰素的需求
Mol Cell Biol. 1990 Aug;10(8):4424-6. doi: 10.1128/mcb.10.8.4424-4426.1990.
2
Oncogenic ras induces an inhibitor of double-stranded RNA-dependent eukaryotic initiation factor 2 alpha-kinase activation.致癌性Ras诱导双链RNA依赖性真核起始因子2α激酶激活的抑制剂。
J Biol Chem. 1992 Nov 15;267(32):23092-8.
3
Direct induction of interferon-gamma- and interferon-alpha/beta-inducible genes by double-stranded RNA.双链RNA直接诱导γ干扰素和α/β干扰素诱导基因
J Interferon Res. 1991 Jun;11(3):131-41. doi: 10.1089/jir.1991.11.131.
4
Double-stranded RNA decreases IGF-I gene expression in a protein kinase R-dependent, but type I interferon-independent, mechanism in C6 rat glioma cells.双链RNA通过一种蛋白激酶R依赖但I型干扰素非依赖的机制降低C6大鼠胶质瘤细胞中IGF-I基因的表达。
Endocrinology. 2002 Feb;143(2):525-34. doi: 10.1210/endo.143.2.8628.
5
Transcriptional induction by double-stranded RNA is mediated by interferon-stimulated response elements without activation of interferon-stimulated gene factor 3.双链RNA介导的转录诱导由干扰素刺激反应元件介导，而不激活干扰素刺激基因因子3。
J Biol Chem. 1995 Aug 18;270(33):19624-9. doi: 10.1074/jbc.270.33.19624.
6
Platelet-derived growth factor signal transduction through the interferon-inducible kinase PKR. Immediate early gene induction.血小板衍生生长因子通过干扰素诱导激酶PKR进行信号转导。立即早期基因诱导。
J Biol Chem. 1995 Feb 17;270(7):3100-6. doi: 10.1074/jbc.270.7.3100.
7
Double-stranded RNA activates novel factors that bind to the interferon-stimulated response element.双链RNA激活与干扰素刺激反应元件结合的新因子。
Mol Cell Biol. 1993 Jun;13(6):3756-64. doi: 10.1128/mcb.13.6.3756-3764.1993.
8
Antisense-fos RNA causes partial reversion of the transformed phenotypes induced by the c-Ha-ras oncogene.反义-fos RNA可使由c-Ha-ras癌基因诱导的转化表型部分逆转。
Mol Cell Biol. 1990 Apr;10(4):1545-55. doi: 10.1128/mcb.10.4.1545-1555.1990.
9
Mitogenic effect of double-stranded RNA in human fibroblasts: role of autogenous interferon.双链RNA对人成纤维细胞的促有丝分裂作用：自身干扰素的作用
J Cell Physiol. 1987 Jan;130(1):37-43. doi: 10.1002/jcp.1041300107.
10
Implication of double-stranded RNA signaling in the etiology of autoimmune myasthenia gravis.双链 RNA 信号在自身免疫性重症肌无力发病机制中的意义。
Ann Neurol. 2013 Feb;73(2):281-93. doi: 10.1002/ana.23791. Epub 2012 Dec 31.

本文引用的文献

1
Selective reduction of c-myc mRNA in Daudi cells by human beta interferon.人β干扰素对Daudi细胞中c-myc信使核糖核酸的选择性降低作用
Proc Natl Acad Sci U S A. 1984 Mar;81(6):1747-50. doi: 10.1073/pnas.81.6.1747.
2
Phosphoproteins altered by antiproliferative doses of human interferon- beta in a human bladder carcinoma cell line.
Biochem Biophys Res Commun. 1984 Mar 30;119(3):941-8. doi: 10.1016/0006-291x(84)90864-7.
3
Biochemical correlates of phenotypic reversion in interferon-treated mouse cells transformed by a human oncogene.经人癌基因转化的干扰素处理小鼠细胞中表型逆转的生化关联
Biochem Biophys Res Commun. 1984 Feb 29;119(1):21-8. doi: 10.1016/0006-291x(84)91612-7.
4
Mouse cells contain two distinct ras gene mRNA species that can be translated into a p21 onc protein.小鼠细胞含有两种不同的ras基因mRNA种类，它们可被翻译成p21癌蛋白。
Mol Cell Biol. 1982 Nov;2(11):1339-45. doi: 10.1128/mcb.2.11.1339-1345.1982.
5
Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene.对3T3细胞的刺激会诱导原癌基因c-fos的转录。
Nature. 1984;311(5985):433-8. doi: 10.1038/311433a0.
6
Viral infection and host defense.病毒感染与宿主防御。
Science. 1974 Dec 27;186(4170):1172-8. doi: 10.1126/science.186.4170.1172.
7
Double-stranded poliovirus RNA inhibits initiation of protein synthesis by reticulocyte lysates.双链脊髓灰质炎病毒RNA抑制网织红细胞裂解物的蛋白质合成起始。
Proc Natl Acad Sci U S A. 1971 May;68(5):1075-8. doi: 10.1073/pnas.68.5.1075.
8
Differential regulation of interferon-induced mRNAs and c-myc mRNA by alpha- and gamma-interferons.α干扰素和γ干扰素对干扰素诱导的mRNA和c-myc mRNA的差异调节
Eur J Biochem. 1985 Dec 2;153(2):367-71. doi: 10.1111/j.1432-1033.1985.tb09312.x.
9
Mitogenic effect of double-stranded RNA in human fibroblasts: role of autogenous interferon.双链RNA对人成纤维细胞的促有丝分裂作用：自身干扰素的作用
J Cell Physiol. 1987 Jan;130(1):37-43. doi: 10.1002/jcp.1041300107.
10
Characterization of the double-stranded RNA implicated in the inhibition of protein synthesis in cells infected with a mutant adenovirus defective for VA RNA.对感染了VA RNA缺陷型突变腺病毒的细胞中涉及蛋白质合成抑制的双链RNA的特性分析。
Virology. 1988 May;164(1):106-13. doi: 10.1016/0042-6822(88)90625-3.