Tacha David, Bremer Ryan, Haas Thomas, Qi Weiman
From the Department of Research and Development, Biocare Medical, LLC, Concord, California (Drs Tacha, Bremer, and Qi); and the Department of Pathology, Mercy General Hospital, Janesville, Wisconsin (Dr Haas).
Arch Pathol Lab Med. 2014 Oct;138(10):1358-64. doi: 10.5858/arpa.2013-0342-OA. Epub 2014 Feb 14.
Immunohistochemistry is important to the pathologist for accurate diagnosis of lung cancer. In recent studies, a rabbit polyclonal p40 (RPp40) antibody demonstrated equivalent staining versus anti-p63 in lung squamous cell carcinoma, and superior specificity because it stains a lesser percentage of lung adenocarcinoma.
To develop an anti-p40 mouse monoclonal antibody (MMp40) for immunohistochemistry, and to evaluate its sensitivity and specificity in normal and neoplastic tissues, with emphasis on lung cancer.
The MMp40 (BC28) antibody was developed and tested for specificity and sensitivity on normal (n = 34) and neoplastic tissues (n = 493). Staining of MMp40, p63, and RPp40 were directly compared in lung cancers, and MMp40 was evaluated in breast, bladder, skin, prostate, and head and neck cancers. Benign prostate glands and prostatic intraepithelial neoplasia were also tested in a direct comparison of MMp40 versus p63.
The MMp40 provided equivalent staining versus RPp40 and p63 in lung squamous cell carcinoma, but stained a lesser percentage of lung adenocarcinoma than p63 did. The MMp40 staining was observed in a greater percentage of urothelial carcinomas, squamous and basal cell skin cancers, and head and neck cancers of squamous cell origin. No breast-infiltrating ductal carcinomas or prostatic adenocarcinomas were stained. The MMp40 expression in basal cells of prostate glands and prostatic intraepithelial neoplasia were almost identical to those of p63.
The MMp40 (BC28) monoclonal antibody is a high-quality screening antibody for determining squamous cell carcinoma in lung cancers, skin cancers of squamous or basal cell origin, squamous cell head and neck cancers, and urothelial carcinomas.
免疫组织化学对于病理学家准确诊断肺癌至关重要。在最近的研究中，一种兔多克隆p40（RPp40）抗体在肺鳞状细胞癌中的染色效果与抗p63相当，且特异性更高，因为它对肺腺癌的染色比例更低。
开发一种用于免疫组织化学的抗p40小鼠单克隆抗体（MMp40），并评估其在正常组织和肿瘤组织中的敏感性和特异性，重点是肺癌。
开发了MMp40（BC28）抗体，并在正常组织（n = 34）和肿瘤组织（n = 493）上测试其特异性和敏感性。在肺癌中直接比较MMp40、p63和RPp40的染色情况，并在乳腺癌、膀胱癌、皮肤癌、前列腺癌和头颈癌中评估MMp40。还在MMp40与p63的直接比较中测试了良性前列腺腺泡和前列腺上皮内瘤变。
在肺鳞状细胞癌中，MMp40的染色效果与RPp40和p63相当，但对肺腺癌的染色比例低于p63。在更高比例的尿路上皮癌、鳞状和基底细胞皮肤癌以及鳞状细胞起源的头颈癌中观察到MMp40染色。未观察到乳腺浸润性导管癌或前列腺腺癌染色。前列腺腺泡和前列腺上皮内瘤变的基底细胞中MMp40的表达与p63几乎相同。
MMp40（BC28）单克隆抗体是一种高质量的筛查抗体，用于确定肺癌、鳞状或基底细胞起源的皮肤癌、鳞状细胞头颈癌和尿路上皮癌中的鳞状细胞癌。
