Department of Biological Engineering, Utah State University, Logan 84322, UT, USA.
Department of Biological Engineering, Utah State University, Logan 84322, UT, USA.
Anal Chim Acta. 2014 Feb 27;813:90-6. doi: 10.1016/j.aca.2014.01.024. Epub 2014 Jan 16.
The development of a miniaturized and low-cost platform for the highly sensitive, selective and rapid detection of multiplexed metabolites is of great interest for healthcare, pharmaceuticals, food science, and environmental monitoring. Graphene is a delicate single-layer, two-dimensional network of carbon atoms with extraordinary electrical sensing capability. Microfluidic paper with printing technique is a low cost matrix. Here, we demonstrated the development of graphene-ink based biosensor arrays on a microfluidic paper for the multiplexed detection of different metabolites, such as glucose, lactate, xanthine and cholesterol. Our results show that the graphene biosensor arrays can detect multiple metabolites on a microfluidic paper sensitively, rapidly and simultaneously. The device exhibits a fast measuring time of less than 2 min, a low detection limit of 0.3 μM, and a dynamic detection range of 0.3-15 μM. The process is simple and inexpensive to operate and requires a low consumption of sample volume. We anticipate that these results could open exciting opportunities for a variety of applications.
开发一种用于高度敏感、选择性和快速检测多种代谢物的微型化和低成本平台，对于医疗保健、制药、食品科学和环境监测具有重要意义。石墨烯是一种由碳原子组成的精细单层二维网络，具有非凡的电传感能力。基于印刷技术的微流控纸是一种低成本基质。在这里，我们展示了基于石墨烯墨水的生物传感器阵列在微流控纸上的开发，用于同时检测不同代谢物，如葡萄糖、乳酸、黄嘌呤和胆固醇。我们的结果表明，石墨烯生物传感器阵列可以在微流控纸上灵敏、快速、同时地检测多种代谢物。该设备的测量时间不到 2 分钟，检测限低至 0.3 μM，动态检测范围为 0.3-15 μM。该过程操作简单、经济实惠，且需要的样本量很少。我们预计这些结果将为各种应用开辟令人兴奋的机会。
