State Key Laboratory of Food Science and Technology, School of Chemical Engineering, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, China.
College of Veterinary Medicine, Gyeongsang National University, Jinju, Gyeongnam 660-701, Republic of Korea.
Carbohydr Polym. 2014 Mar 15;103:319-24. doi: 10.1016/j.carbpol.2013.12.044. Epub 2013 Dec 21.
Medicinal mushroom polysaccharides such as Ganoderma lucidum polysaccharides (GLPs) have been commonly hypothesized to suppress tumor cells proliferation through immune effects. To verify this hypothesis through investigating comprehensive miRNA expression in polysaccharide treated cancer cells, an anticancer mycelia GLP was employed to disclose miRNA differential expression of human hepatocarcinoma cells (HepG2), by using a miRNA microarray assay based on Sanger miR-Base Release 16. The experiment and the analysis result indicates that among the 61 differential expressed miRNAs (p ≤ 0.01), 17 of them were regulated significantly. GLP can inhibit HepG2 cells directly through regulation of hepatocarcinoma genes. A newly found miR-3131 exhibited the strongest upregulation (92-folds, Log2 = 6.53, p = 0.000016). The miRNAs responded synergistically in both hepatocarcinoma and immune-related aspects.
药用蘑菇多糖，如灵芝多糖 (GLP)，通常被假设通过免疫作用抑制肿瘤细胞增殖。为了通过研究多糖处理的癌细胞中的综合 miRNA 表达来验证这一假设，采用 miRNA 微阵列分析基于桑格 miR-Base Release 16，使用抗肝癌菌丝 GLP 来揭示人肝癌细胞 (HepG2) 的 miRNA 差异表达。实验和分析结果表明，在 61 个差异表达的 miRNA 中（p ≤ 0.01），有 17 个受到显著调控。GLP 可以通过调节肝癌基因直接抑制 HepG2 细胞。新发现的 miR-3131 表现出最强的上调（92 倍，Log2 = 6.53，p = 0.000016）。miRNA 在肝癌和免疫相关方面协同反应。
