Sipping Marius Trésor Kemegne, Mediesse Francine Kengne, Kenmogne Larissa V, Kanemoto Judith Emery Ngomoyogoli, Njamen Dieudonné, Boudjeko Thaddée
Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre, Nkolbisson, University of Yaoundé 1, P.O. Box 17673, Etetak, Yaoundé, Cameroon.
Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé 1, P.O. Box 812, Yaoundé, Cameroon.
Evid Based Complement Alternat Med. 2022 Apr 12;2022:8198859. doi: 10.1155/2022/8198859. eCollection 2022.
Hepatocellular carcinoma (HCC) is one of the most common and lethal diseases worldwide. Its treatment remains ineffective and the prognosis remains severe, thus favoring the emergence of a preventive approach. Mushroom-derived polysaccharides offer great opportunities because of their less toxicity and bioactivities. The present study aimed to investigate the chemopreventive effects of water-soluble polysaccharides from on HCC. Two polysaccharide-rich fractions (GRP I and GRP II) were obtained following hot water and alcohol precipitation. Their proteins, phenol compounds, and total neutral sugar content were assayed. The antiproliferative effect was assessed in MDA-MB 231, Hela, and HepG using the MTT assay. Further, for the study, seven groups of nine rats each received N-diethylnitrosamine (100 mg/kg BW), vehicle (NaCl 0.9%), doxorubicin (10 mg/kg BW), or polysaccharides (125 and 250 mg/kg BW). Liver cancer initiation and progression was assessed by evaluating histomorphology of liver section, hepatic injury markers, hematology, cytokines/chemokines levels, and stress oxidative markers. GRP II presented higher protein and sugar and lower phenol compound content than GRP I. GRP exhibited CC of 340 and 261.7 in HepG cells after 48 h. Moreover, GRP I and GRP II (125 and 250 mg/kg) prevented the alteration of the histoarchitecture of the liver induced by the DEN. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alpha-fetoprotein (AFP), proinflammatory cytokines (G-CSF, IFN, and TNF), and chemokines (eotaxin and fractalkine) levels were significantly decreased in the GRP I- and GRP II-treated groups, while anti-inflammatory cytokines (IL-10 and IL-12p70) levels were increased. The antioxidant defense was also stimulated by reducing malondialdehyde (MDA) and nitric oxide (NO) levels, increasing catalase (CAT) and superoxide dismutase (SOD) activities, and reducing glutathione (GSH) levels. Our results indicate that GRP I exhibits chemopreventive effects by inhibiting cell proliferation and restoring liver architecture, antioxidant enzymes, and cytokines/chemokines balance.
肝细胞癌（HCC）是全球最常见且致命的疾病之一。其治疗效果仍然不佳，预后依然严峻，因此预防方法的出现备受青睐。蘑菇衍生的多糖因其低毒性和生物活性提供了巨大的机会。本研究旨在探讨[具体来源未提及]的水溶性多糖对HCC的化学预防作用。通过热水和乙醇沉淀获得了两个富含多糖的组分（GRP I和GRP II）。测定了它们的蛋白质、酚类化合物和总中性糖含量。使用MTT法在MDA - MB 231、Hela和HepG细胞中评估了其抗增殖作用。此外，在研究中，七组每组九只大鼠分别接受N - 二乙基亚硝胺（100mg/kg体重）、赋形剂（0.9%氯化钠）、阿霉素（10mg/kg体重）或[具体来源未提及]多糖（125和250mg/kg体重）。通过评估肝脏切片的组织形态学、肝损伤标志物、血液学、细胞因子/趋化因子水平和氧化应激标志物来评估肝癌的起始和进展。GRP II的蛋白质和糖含量高于GRP I，酚类化合物含量低于GRP I。48小时后，GRP在HepG细胞中的半数细胞毒性浓度（CC）分别为340和261.7。此外，GRP I和GRP II（125和250mg/kg）可预防由二乙基亚硝胺（DEN）诱导的肝脏组织结构改变。在GRP I和GRP II处理组中，丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、甲胎蛋白（AFP）、促炎细胞因子（粒细胞集落刺激因子、干扰素和肿瘤坏死因子）和趋化因子（嗜酸性粒细胞趋化因子和 fractalkine）水平显著降低，而抗炎细胞因子（白细胞介素 - 10和白细胞介素 - 12p70）水平升高。通过降低丙二醛（MDA）和一氧化氮（NO）水平、增加过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性以及降低谷胱甘肽（GSH）水平，抗氧化防御也得到了刺激。我们的结果表明，GRP I通过抑制细胞增殖和恢复肝脏结构、抗氧化酶以及细胞因子/趋化因子平衡发挥化学预防作用。
