Department of Food Engineering and Bionanocomposite Research Institute, Mokpo National University, 61 Dorimri, Chungkyemyon, Muangun, 534-729 Jeonnam, Republic of Korea.
Department of Food Engineering and Bionanocomposite Research Institute, Mokpo National University, 61 Dorimri, Chungkyemyon, Muangun, 534-729 Jeonnam, Republic of Korea.
Carbohydr Polym. 2014 Mar 15;103:456-65. doi: 10.1016/j.carbpol.2013.12.075. Epub 2014 Jan 5.
Silver nanoparticles (AgNPs) were prepared by a laser ablation method and composite films with the AgNPs and agar were prepared by solvent casting method. UV-vis absorbance test and transmission electron microscopy (TEM) analysis results revealed that non-agglomerated spherical AgNPs were formed by the laser ablation method. The surface color of the resulting agar/AgNPs films exhibited the characteristic plasmonic effect of the AgNPs with the maximum absorption peaks of 400-407 nm. X-ray diffraction (XRD) test results also exhibited characteristic AgNPs crystals with diffraction peaks observed at 2θ values of 38.39°, 44.49°, and 64.45°, which were corresponding to (111), (200), and (220) crystallographic planes of face-centered cubic (fcc) silver crystals, respectively. Thermogravimetric analysis (TGA) results showed that thermal stability of the agar/AgNPs composite films was increased by the inclusion of metallic silver. Water vapor barrier properties and surface hydrophobicity of the agar/AgNPs films increased slightly with the increase in AgNPs content but they were not statistically significant (p>0.05), while mechanical strength and stiffness of the composite films decreased slightly (p<0.05). The agar/AgNPs films exhibited distinctive antimicrobial activity against both Gram-positive (Listeria monocytogenes) and Gram-negative (Escherichia coli O157:H7) bacterial pathogens.
采用激光烧蚀法制备了银纳米粒子（AgNPs），并通过溶剂浇铸法制备了AgNPs 和琼脂的复合薄膜。紫外-可见吸收测试和透射电子显微镜（TEM）分析结果表明，激光烧蚀法制备的 AgNPs 为非团聚的球形。所得琼脂/AgNPs 薄膜的表面颜色呈现出 AgNPs 的等离子体效应特征，最大吸收峰位于 400-407nm。X 射线衍射（XRD）测试结果也表现出特征 AgNPs 晶体，在 2θ 值为 38.39°、44.49°和 64.45°处观察到衍射峰，分别对应于面心立方（fcc）银晶体的（111）、（200）和（220）晶面。热重分析（TGA）结果表明，金属银的加入提高了琼脂/AgNPs 复合薄膜的热稳定性。琼脂/AgNPs 薄膜的水蒸气阻隔性能和表面疏水性随着 AgNPs 含量的增加略有增加，但无统计学意义（p>0.05），而复合薄膜的机械强度和刚性略有降低（p<0.05）。琼脂/AgNPs 薄膜对革兰氏阳性（单核细胞增生李斯特菌）和革兰氏阴性（大肠杆菌 O157:H7）细菌病原体均表现出独特的抗菌活性。
