Namık Kemal University, Faculty of Science and Arts, Chemistry Department, Biochemistry Division, Tekirdağ, Turkey.
Namık Kemal University, Faculty of Science and Arts, Chemistry Department, Biochemistry Division, Tekirdağ, Turkey.
Anal Chim Acta. 2014 Mar 3;814:31-8. doi: 10.1016/j.aca.2014.01.041. Epub 2014 Jan 23.
We have developed a new immunological biosensor for ultrasensitive quantification of human epidermal growth factor receptor-3(HER-3). In order to construct the biosensor, the gold electrode surface was layered with, hexanedithiol, gold nanoparticles, and cysteamine, respectively. Anti-HER-3 antibody was covalently attached to cysteamine by glutaraldehyde and used as a bioreceptor in a biosensor system for the first time by this study. Surface characterization was obtained by means of electrochemical impedance spectroscopy and voltammetry. The proposed biosensor showed a good analytical performance for the detection of HER-3 ranging from 0.2 to 1.4 pg mL(-1). Kramers-Kronig transform was performed on the experimental impedance data. Moreover, in an immunosensor system, the single frequency impedance technique was firstly used for characterization of interaction between HER-3 and anti-HER-3. Finally the presented biosensor was applied to artificial serum samples spiked with HER-3.
我们开发了一种新的免疫生物传感器，用于超灵敏定量检测人表皮生长因子受体-3（HER-3）。为了构建该生物传感器，金电极表面分别依次沉积了己二硫醇、金纳米粒子和半胱胺。本研究首次通过戊二醛将抗 HER-3 抗体共价连接到半胱胺上，并将其用作生物传感器系统中的生物受体。通过电化学阻抗谱和伏安法获得了表面特征。所提出的生物传感器在检测 HER-3 方面表现出良好的分析性能，检测范围为 0.2 至 1.4 pg mL(-1)。对实验阻抗数据进行了凯拉姆斯-克朗尼克变换。此外，在免疫传感器系统中，首次使用单频阻抗技术来表征 HER-3 与抗 HER-3 之间的相互作用。最后，将所提出的生物传感器应用于人工血清样品中 HER-3 的加标检测。
