Asav Engin, Sezgintürk Mustafa Kemal
Kırklareli University, School of Health, Kırklareli, Turkey.
Namık Kemal University, Faculty of Science, Chemistry Department, Biochemistry Division, Tekirdağ, Turkey.
Int J Biol Macromol. 2014 May;66:273-80. doi: 10.1016/j.ijbiomac.2014.02.032. Epub 2014 Feb 20.
A specific and sensitive biosensor was developed successfully for quantitative detection of human epidermal growth factor receptor by electrochemical impedance spectroscopy. Anti-human epidermal growth factor receptor antibody was covalently immobilized onto a screen-printed carbon electrode modified with a carbon nanotube. Immobilization steps were characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (SEM). After human epidermal growth factor receptor ligates with anti-human epidermal growth factor receptor immobilized onto an electrode surface, charge transfer resistance changes considerably. This electrochemical response was correlated with human epidermal growth factor receptor concentration. Under optimal conditions, the proposed biosensor could detect human epidermal growth factor receptor 2 fg/mL with a linear range from 2 to 14 fg/mL, showing high sensitivity. Kramers-Kronig Transform was performed on the experimental impedance data. Meanwhile, in a biosensor system, the Single Frequency Impedance technique was first used for characterization of interaction between human epidermal growth factor receptor and anti-human epidermal growth factor receptor. Eventually, the proposed biosensor was applied to artificial serum samples spiked with human epidermal growth factor receptor.
通过电化学阻抗谱成功开发了一种用于定量检测人表皮生长因子受体的特异性灵敏生物传感器。将抗人表皮生长因子受体抗体共价固定在经碳纳米管修饰的丝网印刷碳电极上。通过循环伏安法（CV）、电化学阻抗谱（EIS）和扫描电子显微镜（SEM）对固定步骤进行了表征。人表皮生长因子受体与固定在电极表面的抗人表皮生长因子受体结合后，电荷转移电阻发生显著变化。这种电化学响应与人表皮生长因子受体浓度相关。在最佳条件下，所提出的生物传感器能够检测出人表皮生长因子受体，检测限为2 fg/mL，线性范围为2至14 fg/mL，显示出高灵敏度。对实验阻抗数据进行了克喇末-克朗尼格变换。同时，在生物传感器系统中，首次使用单频阻抗技术表征人表皮生长因子受体与抗人表皮生长因子受体之间的相互作用。最终，将所提出的生物传感器应用于添加了人表皮生长因子受体的人工血清样品。
