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利用衰变加速因子mRNA的3'末端序列对T淋巴细胞表面抗原CD8进行糖脂重锚定。

Glycolipid reanchoring of T-lymphocyte surface antigen CD8 using the 3' end sequence of decay-accelerating factor's mRNA.

作者信息

Tykocinski M L, Shu H K, Ayers D J, Walter E I, Getty R R, Groger R K, Hauer C A, Medof M E

机构信息

Institute of Pathology, Case Western Reserve University, Cleveland, OH 44106.

出版信息

Proc Natl Acad Sci U S A. 1988 May;85(10):3555-9. doi: 10.1073/pnas.85.10.3555.

DOI:10.1073/pnas.85.10.3555
PMID:2453063
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC280252/
Abstract

Decay-accelerating factor (DAF) is one of a family of cell-associated proteins that undergo posttranslational modifications in which glycolipid anchoring structures are substituted for membrane-spanning sequences. The signals that direct the covalent substitution reaction in these proteins are unknown. Human DAF was expressed in Chinese hamster ovary (CHO) cells and murine BW lymphocytes. In both cases, the xenogeneic DAF in transfectants incorporated a glycolipid anchor. A chimeric CD8-DAF cDNA, encompassing the extra-cellular region of the T-lymphocyte surface antigen CD8 and the 3' end of DAF mRNA (encoding the C-terminal region of mature DAF as well as the hydrophobic extension peptide), was expressed in human leukemia lines after transfection with an Epstein-Barr virus-based episomal vector. The chimeric protein in transfectants demonstrated glycolipid anchoring, whereas unaltered CD8 in control experiments did not. The signals directing glycolipid anchoring in eukaryotic cells are thus evolutionarily conserved and contained in the 3' end of the DAF sequence.

摘要

衰变加速因子（DAF）是细胞相关蛋白家族的一员，这些蛋白会经历翻译后修饰，其中糖脂锚定结构取代了跨膜序列。指导这些蛋白中发生共价取代反应的信号尚不清楚。人DAF在中国仓鼠卵巢（CHO）细胞和小鼠BW淋巴细胞中表达。在这两种情况下，转染细胞中的异种DAF都掺入了糖脂锚。一个嵌合的CD8-DAF cDNA，包含T淋巴细胞表面抗原CD8的细胞外区域和DAF mRNA的3'端（编码成熟DAF的C末端区域以及疏水延伸肽），在用基于爱泼斯坦-巴尔病毒的附加型载体转染后人白血病细胞系中表达。转染细胞中的嵌合蛋白表现出糖脂锚定，而对照实验中未改变的CD8则没有。因此，指导真核细胞中糖脂锚定的信号在进化上是保守的，并且包含在DAF序列的3'端。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/667d/280252/5464fe43257f/pnas00262-0296-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/667d/280252/d5c1ad56baef/pnas00262-0295-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/667d/280252/5464fe43257f/pnas00262-0296-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/667d/280252/d5c1ad56baef/pnas00262-0295-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/667d/280252/5464fe43257f/pnas00262-0296-a.jpg

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