Sandri-Goldin R M, Goldin A L, Levine M, Glorioso J
Methods Enzymol. 1983;101:402-11. doi: 10.1016/0076-6879(83)01030-7.
Protoplast fusion is a highly efficient method for effecting gene transfer to cells in culture resulting in stable transformation at high frequency. A number of cell lines have been used successfully as recipients. There is no need to isolate and purify the DNA, which not only saves time and effort but eliminates steps that cause nicking or breaking of large cloned inserts. The high-frequency transformation achievable by protoplast fusion should make this procedure useful for studies on gene expression and for screening cloned genomic libraries for genes that can be expressed in recipient cells.
原生质体融合是一种高效的方法，可将基因导入培养细胞，从而实现高频稳定转化。许多细胞系已成功用作受体。无需分离和纯化DNA，这不仅节省了时间和精力，还省去了导致大的克隆插入片段切口或断裂的步骤。原生质体融合可实现的高频转化应使该方法可用于基因表达研究以及筛选可在受体细胞中表达的基因的克隆基因组文库。
