Griesinger Hans, Fuchs Beate, Süß Rosmarie, Matheis Katerina, Schulz Michael, Schiller Jürgen
Merck KGaA, D-64293 Darmstadt, Germany.
Institute of Medical Physics and Biophysics, Faculty of Medicine, University of Leipzig, D-04107 Leipzig, Germany.
Anal Biochem. 2014 Apr 15;451:45-7. doi: 10.1016/j.ab.2014.02.002. Epub 2014 Feb 11.
Normal phase thin-layer chromatography (NP TLC) is an established method of (phospho)lipid analysis. The determination of the fatty acyl composition is, however, a more challenging task by NP TLC. The direct coupling of TLC separation with mass spectrometric detection (e.g., matrix-assisted laser desorption/ionization mass spectrometry, MALDI MS), however, enables a detailed characterization of complex lipid mixtures. Here we show that the thickness of the silica gel layer has a considerable effect on the quality of the mass spectra recorded directly from the TLC plate. In particular, the intensity of the matrix background signals can be reduced if "thinner" TLC layers are used.
正相薄层色谱法（NP TLC）是一种成熟的（磷酸）脂质分析方法。然而，通过NP TLC测定脂肪酰基组成是一项更具挑战性的任务。然而，将TLC分离与质谱检测（例如，基质辅助激光解吸/电离质谱，MALDI MS）直接联用，能够对复杂脂质混合物进行详细表征。在此我们表明，硅胶层的厚度对直接从TLC板记录的质谱质量有相当大的影响。特别是，如果使用“更薄”的TLC层，基质背景信号的强度可以降低。
