Soejima Mikiko, Koda Yoshiro
Department of Forensic Medicine and Human Genetics, Kurume University School of Medicine, Kurume 830-0011, Japan.
Department of Forensic Medicine and Human Genetics, Kurume University School of Medicine, Kurume 830-0011, Japan.
Forensic Sci Int. 2014 Apr;237:27-9. doi: 10.1016/j.forsciint.2014.01.008. Epub 2014 Jan 26.
We assessed the technical performance and robustness of the point-of-care test for C-reactive protein (CRP) NycoCard CRP for use in forensic autopsy cases. The results of 17 of 39 cadaver blood samples that had CRP in the range effectively measured by the NycoCard (5-120mg/l) correlated well (r=0.99) with those of quantitative latex agglutination immunoassay (turbidimetry), and the out-of-range NycoCard results were fully consistent with those obtained by turbidimetry. For the ten sera whose CRP >120mg/l according to NycoCard, a significant correlation (r=0.98) was observed between values multiplied by the dilution ratio and those of turbidimetry. No significant differences were observed after a freeze-thaw procedure. In addition, CRP results using recombinant human CRP spiked with hemoglobin up to 80g/l were not significantly different from the unspiked results in PBS. The test allows reliable and cost-effective on-site measurement of CRP from a small volume of serum (5μl) with simple equipment. This semi-quantification method of CRP should be useful for diagnosis during autopsy.
我们评估了用于法医尸检案例的即时检测C反应蛋白（CRP）的NycoCard CRP检测方法的技术性能和稳健性。39份尸体血样中有17份的CRP浓度在NycoCard有效检测范围内（5 - 120mg/l），其结果与定量乳胶凝集免疫测定法（比浊法）的结果相关性良好（r = 0.99），且NycoCard超出范围的结果与比浊法获得的结果完全一致。对于NycoCard检测CRP>120mg/l的10份血清，乘以稀释倍数后的值与比浊法的值之间观察到显著相关性（r = 0.98）。冻融处理后未观察到显著差异。此外，血红蛋白浓度高达80g/l的重组人CRP添加到血清中后，其CRP检测结果与未添加的PBS中的结果无显著差异。该检测方法可使用简单设备从小体积血清（5μl）中可靠且经济高效地进行现场CRP检测。这种CRP半定量方法在尸检诊断中应会很有用。
