Wang Xin-Zhi, Liang Jing-Yu, Wen Hong-Mei, Shan Chen-Xiao, Liu Rui
College of Pharmacy, Nanjing University of Chinese Medicine, Xianlin Avenue No. 138, Nanjing 210023, China.
Department of Natural Medicinal Chemistry, China Pharmaceutical University, Tongjia Lane No. 24, Nanjing 210009, China.
J Pharm Biomed Anal. 2014 Jun;94:1-11. doi: 10.1016/j.jpba.2014.01.024. Epub 2014 Jan 26.
Peperomia dindygulensis, with secolignans (SLs) as major bioactive constituents, is a commonly used traditional folk medicine in mainland China for treatment of stomach, liver, mammary, and esophageal cancers. However, to date, there is no method available for the qualitative and quantitative analyses of SLs in this medicinal plant. The purpose of this study was to establish a sensitive, selective, and reproducible method for rapidly profiling, identifying, and determining SLs in the whole plant of P. dindygulensis. Ultra high-performance liquid chromatography (UHPLC) coupled with ultraviolet detector (UV) and quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS) were used for this analyses. The fragmentation behaviors of different types of SLs were described. A total of thirteen SLs, including two new derivatives, were identified or tentatively characterized in P. dindygulensis samples. In addition, seven major SLs in herbal samples from different regions in China were successfully determined. The method developed in this study is suitable for the qualitative and quantitative analyses of SLs in P. dindygulensis, and may be applicable for determining or identifying SLs from other Pepermia genus plants.
丁迪古椒草以裂环木脂素（SLs）作为主要生物活性成分，是中国大陆一种常用的传统民间药物，用于治疗胃癌、肝癌、乳腺癌和食管癌。然而，迄今为止，尚无方法可对这种药用植物中的裂环木脂素进行定性和定量分析。本研究的目的是建立一种灵敏、选择性好且可重复的方法，用于快速分析、鉴定和测定丁迪古椒草全株中的裂环木脂素。采用超高效液相色谱（UHPLC）结合紫外检测器（UV）和四极杆串联飞行时间质谱（Q-TOF-MS）进行该分析。描述了不同类型裂环木脂素的裂解行为。在丁迪古椒草样品中鉴定或初步表征了总共13种裂环木脂素，包括两种新衍生物。此外，成功测定了中国不同地区草药样品中的7种主要裂环木脂素。本研究建立的方法适用于丁迪古椒草中裂环木脂素的定性和定量分析，可能也适用于测定或鉴定其他草胡椒属植物中的裂环木脂素。
