利用抗稻纵卷叶螟虹彩病毒（虹彩病毒6型）的单克隆抗体鉴定病毒抗原决定簇。简报

Identification of viral antigenic determinants by monoclonal antibodies directed against Chilo iridescent virus (iridovirus type 6). Brief report.

作者信息

Orange N, Guerillon J, Devauchelle G

机构信息

Laboratoire de Microbiologie, CNRS UA 203, Faculté des Sciences et Techniques de Rouen, Mont-Saint-Aignan, France.

出版信息

Arch Virol. 1988;99(3-4):243-8. doi: 10.1007/BF01311073.

DOI:10.1007/BF01311073

PMID:2453186

Abstract

Monoclonal antibodies (McAbs) obtained against Iridovirus type 6 (CIV) were characterized by Western blotting and/or immunoprecipitation. Seven McAbs were found to be strongly reactive with viral polypeptides of molecular weights 16 K and 18 K by Western blotting. Two McAbs were directed against a complex composed of 30 K, 50 K, and 100 K polypeptides, but failed to react with either of these free polypeptides. This finding could explain the faint reactivity of these McAbs in Western blotting and immunoprecipitation. The reactivity of the other McAbs with their antigenic determinants is also discussed.

摘要

通过蛋白质免疫印迹法和/或免疫沉淀法对针对虹彩病毒6型（CIV）获得的单克隆抗体（McAbs）进行了表征。通过蛋白质免疫印迹法发现，七种单克隆抗体与分子量为16K和18K的病毒多肽有强烈反应。两种单克隆抗体针对由30K、50K和100K多肽组成的复合物，但不与这些游离多肽中的任何一种发生反应。这一发现可以解释这些单克隆抗体在蛋白质免疫印迹法和免疫沉淀法中反应较弱的原因。还讨论了其他单克隆抗体与其抗原决定簇的反应性。