苹果酸酶在产油真菌高山被孢霉脂肪酸合成过程中的作用
Role of malic enzyme during fatty acid synthesis in the oleaginous fungus Mortierella alpina.
作者信息
Hao Guangfei, Chen Haiqin, Wang Lei, Gu Zhennan, Song Yuanda, Zhang Hao, Chen Wei, Chen Yong Q
机构信息
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
出版信息
Appl Environ Microbiol. 2014 May;80(9):2672-8. doi: 10.1128/AEM.00140-14. Epub 2014 Feb 14.
Abstract
The generation of NADPH by malic enzyme (ME) was postulated to be a rate-limiting step during fatty acid synthesis in oleaginous fungi, based primarily on the results from research focusing on ME in Mucor circinelloides. This hypothesis is challenged by a recent study showing that leucine metabolism, rather than ME, is critical for fatty acid synthesis in M. circinelloides. To clarify this, the gene encoding ME isoform E from Mortierella alpina was homologously expressed. ME overexpression increased the fatty acid content by 30% compared to that for a control. Our results suggest that ME may not be the sole rate-limiting enzyme, but does play a role, during fatty acid synthesis in oleaginous fungi.
摘要
基于主要聚焦于卷枝毛霉中苹果酸酶（ME）的研究结果，推测在产油真菌的脂肪酸合成过程中，苹果酸酶产生还原型辅酶Ⅱ（NADPH）是一个限速步骤。最近一项研究对这一假说提出了挑战，该研究表明亮氨酸代谢而非苹果酸酶对卷枝毛霉的脂肪酸合成至关重要。为了阐明这一点，来自高山被孢霉的编码苹果酸酶同工型E的基因被同源表达。与对照相比，苹果酸酶过表达使脂肪酸含量增加了30%。我们的结果表明，在产油真菌的脂肪酸合成过程中，苹果酸酶可能不是唯一的限速酶，但确实发挥了作用。
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