Industrial Biosciences, E.I. du Pont de Nemours and Company, Wilmington, Delaware, USA.
Nat Biotechnol. 2013 Aug;31(8):734-40. doi: 10.1038/nbt.2622. Epub 2013 Jul 21.
The availability of the omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) is currently limited because they are produced mainly by marine fisheries that cannot keep pace with the demands of the growing market for these products. A sustainable non-animal source of EPA and DHA is needed. Metabolic engineering of the oleaginous yeast Yarrowia lipolytica resulted in a strain that produced EPA at 15% of dry cell weight. The engineered yeast lipid comprises EPA at 56.6% and saturated fatty acids at less than 5% by weight, which are the highest and the lowest percentages, respectively, among known EPA sources. Inactivation of the peroxisome biogenesis gene PEX10 was crucial in obtaining high EPA yields and may increase the yields of other commercially desirable lipid-related products. This technology platform enables the production of lipids with tailored fatty acid compositions and provides a sustainable source of EPA.
目前，由于 omega-3 脂肪酸二十碳五烯酸 (EPA) 和二十二碳六烯酸 (DHA) 主要由无法满足这些产品日益增长的市场需求的海洋渔业生产，因此供应有限。需要一种可持续的非动物来源的 EPA 和 DHA。通过对产油酵母解脂耶氏酵母进行代谢工程改造，得到了一种 EPA 产量达到干重 15%的菌株。工程酵母脂质中 EPA 的含量为 56.6%，饱和脂肪酸的含量低于 5%，这分别是已知 EPA 来源中最高和最低的百分比。过氧化物酶体生物发生基因 PEX10 的失活对于获得高 EPA 产量至关重要，并且可能会提高其他商业上理想的脂质相关产品的产量。这项技术平台使具有定制脂肪酸组成的脂质的生产成为可能，并为 EPA 提供了可持续的来源。
