通过高山被孢霉中线粒体苹果酸酶的同源过表达增加脂肪酸不饱和度及花生四烯酸的产生。
Increased fatty acid unsaturation and production of arachidonic acid by homologous over-expression of the mitochondrial malic enzyme in Mortierella alpina.
作者信息
Hao Guangfei, Chen Haiqin, Du Kai, Huang Xiaoyun, Song Yuanda, Gu Zhennan, Wang Lei, Zhang Hao, Chen Wei, Chen Yong Q
机构信息
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China.
出版信息
Biotechnol Lett. 2014 Sep;36(9):1827-34. doi: 10.1007/s10529-014-1546-x. Epub 2014 May 27.
Abstract
Malic enzyme (ME) catalyses the oxidative decarboxylation of L-malate to pyruvate and provides NADPH for intracellular metabolism, such as fatty acid synthesis. Here, the mitochondrial ME (mME) gene from Mortierella alpina was homologously over-expressed. Compared with controls, fungal arachidonic acid (ARA; 20:4 n-6) content increased by 60 % without affecting the total fatty acid content. Our results suggest that enhancing mME activity may be an effective mean to increase industrial production of ARA in M. alpina.
摘要
苹果酸酶（ME）催化L-苹果酸氧化脱羧生成丙酮酸，并为细胞内代谢（如脂肪酸合成）提供还原型辅酶II（NADPH）。在此，来自高山被孢霉的线粒体苹果酸酶（mME）基因被同源过表达。与对照相比，真菌花生四烯酸（ARA；20:4 n-6）含量增加了60%，而不影响总脂肪酸含量。我们的结果表明，提高mME活性可能是增加高山被孢霉中ARA工业产量的有效手段。
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