Azevedo C, Oliveira E
Department of Cell Biology, University of Oporto, Portugal.
J Submicrosc Cytol Pathol. 1988 Jan;20(1):81-7.
The technique for ultrastructural localization of argyrophilic proteins was modified and NOR-Silver staining methods were applied to the study of the distribution of these proteins in the spermatic cells of Nucella lapillus (Gastropoda, Prosobranchia). Two types of selective silver deposits were found during the different phases of spermiogenesis and in mature spermatozoa. Argyrophilic nuclear, nucleolar and cytoplasmic proteins were simultaneously detected by improvement of a modified one-step silver technique.
对嗜银蛋白超微结构定位技术进行了改进,并将NOR-银染色方法应用于研究荔枝螺(腹足纲,前鳃亚纲)精细胞中这些蛋白的分布。在精子发生的不同阶段以及成熟精子中发现了两种类型的选择性银沉积。通过改进的一步银技术,同时检测到了嗜银核蛋白、核仁蛋白和细胞质蛋白。