Amikura R M, Yamada H, Hirai S, Nagano H
Department of Anatomy, Tokyo Women's Medical College, Japan.
Gamete Res. 1987 Apr;16(4):291-301. doi: 10.1002/mrd.1120160403.
Changes in intracellular localization of argyrophilic proteins visualized as silver-stained particles by nuclear organizer region (NOR)-silver staining were investigated in starfish oocyte maturation. The silver-stained particles were localized in the germinal vesicle and nucleolus of immature oocytes and dispersed into the cytoplasm at the time of germinal vesicle breakdown (GVBD). In the mature egg cytoplasm, silver-stained particles were distributed on yolk-like granules with diameters of 0.3-1.0 microns. In spermatozoa, silver-stained particles were detected heavily in the acrosome and centrosomes but few were detected in the nucleus, whereas they were present in the male pronucleus of fertilized eggs. The silver-stained particles were removed by pretreatment of eggs with protease but not with nuclease. These results indicate that argyrophilic proteins disperse to the egg cytoplasm during GVBD and might be incorporated to the male pronucleus from the egg cytoplasm in fertilization. The morphological changes from chromosomes through chromosome vesicles to female pronucleus were also observed with light microscopy after NOR-silver staining.
通过核仁组织区（NOR）-银染法将嗜银蛋白的细胞内定位变化可视化为银染颗粒，对海星卵母细胞成熟过程进行了研究。银染颗粒定位于未成熟卵母细胞的生发泡和核仁中，并在生发泡破裂（GVBD）时分散到细胞质中。在成熟卵细胞质中，银染颗粒分布在直径为0.3-1.0微米的类卵黄颗粒上。在精子中，银染颗粒在顶体和中心体中大量检测到，但在细胞核中很少检测到，而它们存在于受精卵的雄原核中。用蛋白酶预处理卵可去除银染颗粒，而用核酸酶预处理则不能。这些结果表明，嗜银蛋白在GVBD期间分散到卵细胞质中，并可能在受精过程中从卵细胞质整合到雄原核中。在NOR-银染后，通过光学显微镜也观察到了从染色体到染色体小泡再到雌原核的形态变化。
