Sarkar R S, Philip J, Prathip Kumar B R, Pathak Amardeep
Commandant, 151 Base Hospital, C/o 99 APO.
Associate Professor, Department of Transfusion Medicine, AFMC, Pune 40, India.
Med J Armed Forces India. 2013 Jan;69(1):37-40. doi: 10.1016/j.mjafi.2012.07.018. Epub 2012 Oct 23.
Fresh Frozen Plasma (FFP) is a blood component prepared from whole blood or from apheresis donation. Donor leukocytes including lymphocytes are present in FFP in significant numbers inspite of freezing, responsible for Transfusion Associated Graft versus Host Disease (TA-GvHD).
75 units of FFP prepared at our centre were analysed. After thawing of FFP a small aliquot was made under sterile conditions and another after irradiating the product. In both the parts, variables of haemostasis were measured in parallel, using automated coagulation analyser. Activated partial thromboplastin time (APTT), prothrombin time (PT), International Normalized Ratio (INR), Thrombin time (TT), coagulation factors FI, FII, FV, FVIII, FIX, FX, FXI, FXII, vWF Ag, inhibitors of coagulation (protein C & S) and d-dimer were measured.
Gamma irradiation of FFP with 30 Gy resulted in weak activation of coagulation system which was evident in the form of shortening of PT, APTT and TT. The activity of coagulation factors FIX, FX, FXI, and FXII were significantly raised after irradiation. No reduction in the activity of inhibitors of coagulation (protein C & S) or increase in d-dimers was observed following irradiation of FFP.
Gamma irradiation of FFP with 30 Gy resulted in a significant but very weak alteration of coagulation system in FFP.
新鲜冰冻血浆（FFP）是一种由全血或单采血液制备的血液成分。尽管经过冷冻，FFP中仍存在大量包括淋巴细胞在内的供体白细胞，这些白细胞会引发输血相关移植物抗宿主病（TA-GvHD）。
对本中心制备的75单位FFP进行分析。FFP解冻后，在无菌条件下取一小份样本，另一小份样本则在对产品进行辐照后获取。在这两份样本中，使用自动凝血分析仪并行测量止血变量。测量活化部分凝血活酶时间（APTT）、凝血酶原时间（PT）、国际标准化比值（INR）、凝血酶时间（TT）、凝血因子FI、FII、FV、FVIII、FIX、FX、FXI、FXII、血管性血友病因子抗原（vWF Ag）、凝血抑制剂（蛋白C和蛋白S）以及D-二聚体。
用30 Gy的γ射线辐照FFP导致凝血系统轻度激活，这在PT、APTT和TT缩短的形式中明显体现。辐照后，凝血因子FIX、FX、FXI和FXII的活性显著提高。FFP辐照后未观察到凝血抑制剂（蛋白C和蛋白S）活性降低或D-二聚体增加。
用30 Gy的γ射线辐照FFP导致FFP中的凝血系统发生显著但非常轻微的改变。
