Department of Animal Sciences, Center for Nutrition and Pregnancy, North Dakota State University, Fargo, North Dakota, USA.
Department of Animal Sciences, Center for Nutrition and Pregnancy, North Dakota State University, Fargo, North Dakota, USA.
Theriogenology. 2014 Apr 15;81(7):887-95. doi: 10.1016/j.theriogenology.2013.12.024. Epub 2014 Jan 7.
The aim of this study was to determine the effects of maternal diet with adequate (A) or high (H) selenium (Se) supplementation on ovarian and uterine characteristics, and onset of puberty in adolescent offspring. Sheep were fed a maintenance (M) diet with ASe or HSe levels from breeding to parturition. From Day 50 to parturition, a portion of the ewes from ASe and HSe groups was fed restricted (R, 60% of M) or excess (E, 140% of M) diet. Immediately after birth, lambs were separated from their dams and given artificial colostrum for 20 hours, followed by milk replacer. From Day 57.3 ± 0.6, ewe lambs were fed a pelleted grower diet until Day 116.3 ± 0.6 when they were transitioned to a finisher diet. From Day 99 to 180, serum samples were collected weekly from jugular vein for progesterone analysis to determine onset of puberty. Reproductive tissues were collected on Day 180.1 ± 0.4 of age. Maternal diet or Se supplementation did not affect uterine or ovarian weight and onset of puberty. However, area under the curve for progesterone was greater (P = 0.05) in ASe compared with HSe groups, and was greater in ASeM than HSeM group. In CLs, labeling index (LI; a proportion of proliferating cells) was less (P < 0.04) in HSeM than ASeM group, and in stroma was less (P < 0.05) in R and E groups than M group. Maternal diet did not affect the LI of any follicle types. For all groups combined, LI was the greatest (P < 0.001) in antral, less in early antral and secondary, and the least in atretic follicles. Our results demonstrate that maternal diet influenced ovarian but not uterine characteristics or onset of puberty. These results indicate that maternal plane of nutrition and/or Se supplementation may have specific effects on reproductive function in offspring.
本研究旨在确定母体饮食中适量（A）或高（H）硒（Se）补充对卵巢和子宫特征以及青春期后代初情期的影响。绵羊在繁殖到分娩期间以维持（M）饮食喂养，其 ASe 或 HSe 水平适中。从第 50 天到分娩，部分来自 ASe 和 HSe 组的母羊被喂食限制（R，M 的 60%）或过量（E，M 的 140%）饮食。羔羊出生后立即与其母羊分开，并给予人工初乳 20 小时，然后用代乳料代替。从第 57.3±0.6 天开始，母羊羔羊被喂食颗粒生长日粮，直到第 116.3±0.6 天，然后过渡到育肥日粮。从第 99 天到 180 天，每周从颈静脉采集血清样本进行孕激素分析，以确定初情期的开始。在第 180.1±0.4 天龄时采集生殖组织。母体饮食或 Se 补充对子宫或卵巢重量和初情期没有影响。然而，ASe 组的孕激素曲线下面积大于 HSe 组（P=0.05），ASeM 组大于 HSeM 组。在黄体中，HSeM 组的标记指数（LI；增殖细胞的比例）小于 ASeM 组（P<0.04），在基质中 R 和 E 组小于 M 组（P<0.05）。母体饮食对任何卵泡类型的 LI 都没有影响。对于所有组的综合分析，LI 在窦状卵泡中最大（P<0.001），在早窦状卵泡和次级卵泡中较小，在闭锁卵泡中最小。我们的研究结果表明，母体饮食影响卵巢但不影响子宫特征或初情期。这些结果表明，母体营养水平和/或 Se 补充可能对后代的生殖功能有特定的影响。
