Szentiks C A, Tsangaras K, Abendroth B, Scheuch M, Stenglein M D, Wohlsein P, Heeger F, Höveler R, Chen W, Sun W, Damiani A, Nikolin V, Gruber A D, Grobbel M, Kalthoff D, Höper D, Czirják G Á, Derisi J, Mazzoni C J, Schüle A, Aue A, East M L, Hofer H, Beer M, Osterrieder N, Greenwood A D
Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Str. 17, 10315 Berlin, Germany.
Institute of Diagnostic Virology, Friedrich-Loeffler-Institute, Südufer 10, 17493 Greifswald - Insel Riems, Germany.
J Comp Pathol. 2014 May;150(4):474-88. doi: 10.1016/j.jcpa.2013.12.005. Epub 2013 Dec 19.
This report describes three possibly related incidences of encephalitis, two of them lethal, in captive polar bears (Ursus maritimus). Standard diagnostic methods failed to identify pathogens in any of these cases. A comprehensive, three-stage diagnostic 'pipeline' employing both standard serological methods and new DNA microarray and next generation sequencing-based diagnostics was developed, in part as a consequence of this initial failure. This pipeline approach illustrates the strengths, weaknesses and limitations of these tools in determining pathogen caused deaths in non-model organisms such as wildlife species and why the use of a limited number of diagnostic tools may fail to uncover important wildlife pathogens.
本报告描述了圈养北极熊（Ursus maritimus）中三起可能相关的脑炎病例，其中两例致死。标准诊断方法未能在任何一例中鉴定出病原体。部分由于最初的失败，开发了一种综合的三阶段诊断“流程”，采用标准血清学方法以及新的基于DNA微阵列和下一代测序的诊断方法。这种流程方法说明了这些工具在确定野生动物等非模式生物中病原体导致的死亡方面的优势、劣势和局限性，以及为何使用有限数量的诊断工具可能无法发现重要的野生动物病原体。
