Miadonna A, Leggieri E, Tedeschi A, Lorini M, Motta G, Lucci G, Froldi M, Marini A, Zanussi C
Department of Medicine, Infectious Diseases and Immunopathology, University of Milan, Italy.
Int Arch Allergy Appl Immunol. 1988;86(1):42-7. doi: 10.1159/000234604.
This study was performed to evaluate mediator (histamine and leukotriene C4) release from cord blood and adult blood basophils, challenged with IgE-independent (calcium ionophore A23187) and IgE-mediated (anti-IgE) stimuli. IgE-independent mediator release was similar in adult blood and umbilical blood basophils. Conversely, the anti-IgE-induced histamine and immunoreactive leukotriene C4 (iLTC4) release was significantly reduced in cord blood basophils. Passive sensitization with an IgE-rich serum was followed by a significant increase in the number of eluted IgE molecules from cord blood basophils and by an increase in IgE-mediated histamine release. iLTC4 production was not affected by passive sensitization of umbilical blood basophils. The IgE-dependent mediator release from cord blood basophils was not correlated with the number of cell-bound IgE. In addition, histamine secretion and leukotriene C4 production from cord blood basophils seem to be independent events.
本研究旨在评估脐带血和成人血嗜碱性粒细胞在受到非IgE依赖性（钙离子载体A23187）和IgE介导（抗IgE）刺激后介质（组胺和白三烯C4）的释放情况。非IgE依赖性介质释放在成人血和脐血嗜碱性粒细胞中相似。相反，抗IgE诱导的组胺和免疫反应性白三烯C4（iLTC4）释放在脐带血嗜碱性粒细胞中显著减少。用富含IgE的血清进行被动致敏后，脐带血嗜碱性粒细胞洗脱的IgE分子数量显著增加，且IgE介导的组胺释放增加。iLTC4的产生不受脐血嗜碱性粒细胞被动致敏的影响。脐带血嗜碱性粒细胞中IgE依赖性介质释放与细胞结合IgE的数量无关。此外，脐带血嗜碱性粒细胞的组胺分泌和白三烯C4产生似乎是独立事件。
