Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, 53-114 Wrocław, Poland.
Food Funct. 2014 Apr;5(4):724-33. doi: 10.1039/c3fo60391a.
In the hen immune system the egg content plays as significant a role in the development of the chick as colostrum does in newborn mammals. One of the most important proteins in this system seems to be the main yolk immunoglobulin IgY. It has been shown that IgY is accompanied by an immunostimulatory polypeptide complex named yolkin. In this report the biological activities of yolkin separated by means of four different procedures are presented. It was shown that yolkin acts as an inducer rather than a modulator of cytokine and nitric oxide release, and does not participate in the protection of cells against destructive effects of reactive oxygen species. However, using the perchloric acid procedure it is possible to obtain a peptide fraction with higher inducing activity, stronger antioxidant properties and ability to decrease the NO level induced by lipopolysaccharide. The results obtained show that it is feasible to select one of the presented methods of yolkin isolation that yields a product of particular activity. The properties of yolk peptides not only indicate their roles in the development of chicks, but can also be useful for the regulation of some immunological disturbances.
在母鸡的免疫系统中，卵内容物在小鸡的发育中起着与初乳在新生哺乳动物中相同的重要作用。在这个系统中，最重要的蛋白质之一似乎是主要的蛋黄免疫球蛋白 IgY。已经表明，IgY 伴随着一种称为 yolkin 的免疫刺激多肽复合物。在本报告中，介绍了通过四种不同程序分离的 yolkin 的生物学活性。结果表明，yolkin 作为细胞因子和一氧化氮释放的诱导剂而不是调节剂起作用，并且不参与细胞免受活性氧破坏性影响的保护。然而，使用高氯酸程序可以获得具有更高诱导活性、更强抗氧化性能和降低脂多糖诱导的 NO 水平的能力的肽级分。所得结果表明，可以选择 yolkin 分离的一种方法，该方法可产生具有特定活性的产物。蛋黄肽的特性不仅表明它们在小鸡发育中的作用，而且还可以用于调节某些免疫失调。
