Zambrowicz A, Zabłocka A, Bobak Ł, Macała J, Janusz M, Polanowski A, Trziszka T
Department of Animal Products Technology and Quality Management, Faculty of Food Sciences, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37, 51-630 Wrocław, Poland.
Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Immunochemistry, R. Weigla 12, 53-114 Wrocław, Poland.
Food Chem. 2017 Sep 1;230:705-711. doi: 10.1016/j.foodchem.2017.03.101. Epub 2017 Mar 18.
A large number of bioactive peptides isolated from natural sources are known to play important physiological roles in the human body. It is possible to use these as alternative therapy agents. One example is yolkin which can be useful as a food supplement, a natural therapeutic agent for preventing and treating cognitive disorders of various origins, preferably in patients with unsatisfactory responses to known therapies. A new simple method of isolation of yolkin based on precipitation with ethanol or acetone was developed. The best precipitation efficiency of both ethanol and acetone was achieved when stirred into the starting material to a final concentration of 70%. These methods preserved the ability of yolkin to stimulate human whole blood cells to release anti-inflammatory cytokines and neurotrophins. At first we indicated that yolkin displayed a potential neuroprotective effect by the ability to stimulate cells to produce pro-survival brain derived neurotrophic factor (BDNF).
已知从天然来源分离出的大量生物活性肽在人体中发挥着重要的生理作用。可以将这些肽用作替代治疗剂。一个例子是蛋黄素，它可用作食品补充剂，是一种预防和治疗各种原因引起的认知障碍的天然治疗剂，尤其适用于对已知疗法反应不佳的患者。开发了一种基于用乙醇或丙酮沉淀的蛋黄素分离新方法。当搅拌到起始材料中至最终浓度为70%时，乙醇和丙酮都能达到最佳沉淀效率。这些方法保留了蛋黄素刺激人全血细胞释放抗炎细胞因子和神经营养因子的能力。起初我们指出，蛋黄素通过刺激细胞产生促生存的脑源性神经营养因子（BDNF）而显示出潜在的神经保护作用。
