Abe R, Hodes R J
Immunology Branch, National Cancer Institute, Bethesda, MD 20892.
J Immunol. 1988 Jun 15;140(12):4132-8.
Monospecific T cell clones have been proven to be powerful tools for the characterization of T cell recognition in many Ag-specific as well as allo-specific T cell responses. In this report, in order to elucidate the mechanism of T cell recognition of minor stimulating locus Ag (Mlsc) determinants, Mlsc-specific cloned T cells were employed together with primary T cell responses to clarify the role of MHC-gene products in Mlsc-specific T cell recognition. The results indicated that T cells recognize Mlsc determinants in conjunction with I-region MHC gene products. Moreover, certain MHC haplotypes (e.g., H-2a and H-2k) appear to function efficiently in the "presentation" of Mlsc, whereas other haplotypes (e.g., H-2b and H-2q) function poorly if at all in presenting Mlsc. Experiments with the use of stimulators derived from F1 hybrids between the low stimulatory H-2b, Mlsc strain, C3H.SW, and a panel of Mlsb, H-2-different or intra-H-2 recombinant strains strongly suggested that expression of E alpha E beta molecules on stimulators plays a critical role for Mlsc stimulation. The functional importance of the E alpha E beta product in Mlsc recognition was further demonstrated by the ability of anti-E alpha monoclonal antibody to inhibit the response of cloned Mlsc-specific T cells. Inhibition of the same Mlsc-specific response by anti-A beta k antibody suggests that the A beta product may also play a role in T cell responses to Mlsc.
单特异性T细胞克隆已被证明是表征许多抗原特异性以及同种异体特异性T细胞应答中T细胞识别的有力工具。在本报告中，为了阐明T细胞对次要刺激位点抗原（Mlsc）决定簇的识别机制，将Mlsc特异性克隆T细胞与原代T细胞应答一起使用，以阐明MHC基因产物在Mlsc特异性T细胞识别中的作用。结果表明，T细胞与I区MHC基因产物共同识别Mlsc决定簇。此外，某些MHC单倍型（例如，H-2a和H-2k）在Mlsc的“呈递”中似乎能有效发挥作用，而其他单倍型（例如，H-2b和H-2q）在呈递Mlsc时即使有作用也很微弱。使用低刺激的H-2b、Mlsc品系C3H.SW与一组Mlsb、H-2不同或H-2内重组品系之间的F1杂种衍生的刺激物进行的实验强烈表明，刺激物上EαEβ分子的表达对Mlsc刺激起关键作用。抗Eα单克隆抗体抑制克隆的Mlsc特异性T细胞应答的能力进一步证明了EαEβ产物在Mlsc识别中的功能重要性。抗Aβk抗体对相同的Mlsc特异性应答的抑制表明，Aβ产物也可能在T细胞对Mlsc的应答中起作用。
