Ryan J J, Mond J J, Finkelman F D
Immunobiology and Transplantation Department, Naval Medical Research Institute, Bethesda, MD 20814-5055.
J Immunol. 1988 Aug 15;141(4):1063-73.
In the course of studying the MHC restriction of minor lymphocyte stimulating (Mls) determinants, we observed that variation in the ability to present Mlsc determinants occurred with stimulator cells from different mouse strains that express the same class II MHC restricting elements; for example, one Iad-bearing strain, C3H.HTG, presented this non-MHC moiety, whereas another, C3H.OH, could not. As another example, the prototype Mlsb nonstimulatory H-2d stimulator cell, BALB/c, was shown to encode Mlsc even though it failed to trigger proliferation across this non-MHC barrier. In contrast, H-2d-compatible DBA/2 stimulator cells were capable of eliciting detectable levels of unprimed responder T cell proliferation across an Mlsc difference. Even when the BALB/c H-2d haplotype was replaced with the fully permissive H-2K halplotype, these BALB.K stimulator cells presented Mlsc (but not MHC) less effectively than H-2K-compatible C3H/HeJ stimulator cells. Analysis of the Mlsc-presenting capacity of stimulator cells obtained from (BALB.K x C3H) F1 x BALB.K first backcross and (BALB.K x C3H)F2 animals indicated that non-MHC-control influencing stimulatory ability of this non-H-2 Ag was multigenic. In addition, the capacity of DBA/2 to present Mlsa determinants more effectively than MHC-identical LT/ChReSv stimulator cells may indicate that the presentation of this Mls specificity is also influenced by non-MHC Ir genes. Thus the Mls phenotype of an animal should be considered the combined result of an Mls structural gene, the MHC haplotype, and multiple non-H-2 regulatory influences.
在研究次要淋巴细胞刺激（Mls）决定簇的MHC限制性过程中，我们观察到，对于表达相同II类MHC限制性元件的不同小鼠品系的刺激细胞，其呈递Mlsc决定簇的能力存在差异；例如，一个携带Iad的品系C3H.HTG能够呈递这种非MHC部分，而另一个品系C3H.OH则不能。再举一个例子，典型的Mlsb非刺激性H-2d刺激细胞BALB/c，尽管未能触发跨越这个非MHC屏障的增殖，但被证明编码Mlsc。相比之下，与H-2d相容的DBA/2刺激细胞能够引发可检测水平的未致敏应答T细胞跨越Mlsc差异的增殖。即使将BALB/c的H-2d单倍型替换为完全许可的H-2K单倍型，这些BALB.K刺激细胞呈递Mlsc（而非MHC）的效率仍低于与H-2K相容的C3H/HeJ刺激细胞。对从（BALB.K×C3H）F1×BALB.K首次回交和（BALB.K×C3H）F2动物获得的刺激细胞的Mlsc呈递能力进行分析表明，影响这种非H-2抗原刺激能力的非MHC调控是多基因的。此外，DBA/2比MHC相同的LT/ChReSv刺激细胞更有效地呈递Mlsa决定簇的能力可能表明，这种Mls特异性的呈递也受非MHC Ir基因影响。因此，动物的Mls表型应被视为Mls结构基因、MHC单倍型和多种非H-2调控影响的综合结果。
