Hilbert D M, Cancro M P
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104.
J Immunol. 1988 Jun 15;140(12):4364-71.
An inbred strain was derived from feral mice that respond poorly to phosphorylcholine (PC) but responded normally to a variety of other Ag. Low responsiveness is inherited as a single recessive autosomal trait, characterized by very low levels of both PC-binding serum antibody and PC-specific B cells. Analyses of PC-reactive clonotypes generated in limiting dilution culture, as well as analyses of PC-specific hybridomas, indicate that this strain expresses L and H chain V regions not previously associated with high affinity murine PC-binding antibodies. Further, sequence analyses suggest gene conversion events occurred within the S107VH gene family among the progenitors of this strain subsequent to their divergence from those of most common laboratory strains.
一个近交系源自野生小鼠，该野生小鼠对磷酸胆碱（PC）反应较差，但对多种其他抗原反应正常。低反应性作为一种单一隐性常染色体性状遗传，其特征是PC结合血清抗体和PC特异性B细胞水平都非常低。对有限稀释培养中产生的PC反应性克隆型的分析，以及对PC特异性杂交瘤的分析表明，该品系表达的轻链和重链V区以前未与高亲和力鼠PC结合抗体相关联。此外，序列分析表明，在该品系的祖细胞与大多数常见实验室品系的祖细胞分化之后，S107VH基因家族中发生了基因转换事件。
