Todd I, Chang S P, Perlmutter R M, Aebersold R, Heusser C H, Hood L, Rittenberg M B
J Immunol. 1984 Mar;132(3):1556-60.
The anti-phosphocholine (PC) memory response of BALB/c mice to PC-KLH contains two groups of antibodies distinguished by fine specificity and by expression of the T15 idiotype that dominates Group I but not Group II anti-PC antibodies. The contribution of V kappa genes to this diversity was investigated by the analysis of L chains from PC-binding hybridoma proteins (PCBHP) representative of Group I and Group II. N-terminal amino acid sequence analysis was performed on the L chains of three independently derived Group II PCBHP up to residue 23 (PCG1-1) or 21 (aPC-111-1 and aPC-12-3). These three sequences differed from each other by only one or two residues, but differed by approximately 50% from the L chains of the Group I-like PC-binding myeloma proteins (PCBMP); the Group II sequences are closely related to V kappa 1-3. Isoelectric focusing analysis was also performed on the L chain of PCG1-1, as well as on L chains from PCBHP typical of Group I antibodies, and from an atypical PCBHP differing from Groups I and II in fine specificity. A Group I PCBHP and the atypical PCBHP expressed L chains related to V kappa 8 and V kappa 24, respectively. The L chains of another Group I PCBHP and of the Group II protein, PCG1-1, appeared different from those found in the PCBMP and from each other. The results indicate a more diverse expression of L chains in the memory anti-PC response than is represented by the PCBMP; both V kappa 8- and V kappa 24-derived L chains (and, presumably, somatic variants), as well as products of additional V kappa genes (V kappa 1-3), appear to be present in the anti-PC memory pool.
BALB/c小鼠对磷酰胆碱-钥孔血蓝蛋白（PC-KLH）的抗磷酰胆碱（PC）记忆反应包含两组抗体，这两组抗体在精细特异性以及T15独特型的表达上有所区别，T15独特型在I组抗PC抗体中占主导，但在II组抗PC抗体中则不然。通过分析代表I组和II组的PC结合杂交瘤蛋白（PCBHP）的轻链，研究了Vκ基因对这种多样性的贡献。对三个独立衍生的II组PCBHP（PCG1-1）或21个残基（aPC-111-1和aPC-12-3）的轻链进行了N端氨基酸序列分析，直至第23个残基。这三个序列彼此之间仅相差一两个残基，但与I组样PC结合骨髓瘤蛋白（PCBMP）的轻链相差约50%；II组序列与Vκ1-3密切相关。还对PCG1-1的轻链以及I组抗体典型的PCBHP轻链和在精细特异性上与I组和II组不同的非典型PCBHP轻链进行了等电聚焦分析。一个I组PCBHP和非典型PCBHP分别表达了与Vκ8和Vκ24相关的轻链。另一个I组PCBHP和II组蛋白PCG1-1的轻链与PCBMP中的轻链不同，且彼此也不同。结果表明，记忆性抗PC反应中轻链的表达比PCBMP所代表的更为多样；抗PC记忆库中似乎同时存在Vκ8和Vκ24衍生的轻链（以及可能的体细胞变体）以及其他Vκ基因（Vκ1-3）的产物。
