Genetic variation of bovine leukemia virus (BLV) after replication in cell culture and experimental animals.

作者信息

Camargos M F, Rajão D S, Leite R C, Stancek D, Heinemann M B, Reis J K P

机构信息

Laboratório Nacional Agropecuário, Ministério da Agricultura, Pecuária e Abastecimento, Pedro Leopoldo, MG, Brasil.

Laboratório de Retroviroses, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.

出版信息

Genet Mol Res. 2014 Mar 17;13(1):1717-23. doi: 10.4238/2014.January.22.11.

DOI:10.4238/2014.January.22.11

PMID:24535910

Abstract

This article reports the selection of bovine leukemia virus (BLV) variants after continuous passage in cell lines or experimental animals. Two wild BLV strains isolated from 2 naturally infected Holstein dairy cows in Brazil (cow codes: 485 and 141) were used for the experimental infection of 1 sheep and FLK cells, and 1 rabbit and CC81 cells. Viral DNA was isolated several months after infection, and env gene nucleotide and amino acid sequences of the "passaged" variants were compared against the 2 original infecting wild strains. The sequences of the original infecting wild strains were not recovered after their replication in the cell lines or experimental animals. These results indicate that genetic variation occurred after BLV replication in vivo and in vitro, with new variants being selected.

摘要