Friedman H, Blanchard D K, Newton C, Klein T, Stewart W, Keler T, Nowotny A
Department of Medical Microbiology and Immunology, University of South Florida College of Medicine, Tampa 33620.
J Biol Response Mod. 1987 Dec;6(6):664-77.
Endotoxin derived from Serratia marcescens, as well as a hydrolyzed polysaccharide-rich derivative from the bacteria similar to the White-type polysaccharide (WPS), and hydrolyzed split products of endotoxin, including Lipid A and the nontoxic polysaccharide (PS), were studied in terms of their ability to induce mitogenic proliferation of murine spleen cells in vitro and enhancement of antibody formation to sheep erythrocytes. The intact endotoxin and the Lipid A component induced marked proliferative activity of normal lymphoid cells, but the PS components obtained after a 30 min or longer mild hydrolysis of the endotoxin had much less mitogenic activity. The WPS preparation retained mitogenic activity after 30 min but lost it during continued hydrolysis. Nevertheless, all of the components were marked adjuvants for murine spleen cells in vitro in terms of enhancing the antibody response to sheep red cells. Both the intact endotoxin as well as the Lipid A component induced interleukin-1 and interferons, including alpha/beta and gamma, following stimulation of spleen cell cultures for 24 h in vitro. Although only the endotoxin and Lipid A, but not the PS components, were mitogenic for murine splenocytes, all of these preparations had immune adjuvant effects and induced spleen cells to produce or release interferons. However, the PS-rich derivative did not induce tumor necrosis factor. These distinct properties of components of endotoxin indicate that the nontoxic PS-rich derivative, as compared to the toxic endotoxin or Lipid A, has some immunostimulatory activities but lacks others.
对源自粘质沙雷氏菌的内毒素、该细菌的一种富含水解多糖的类似白色型多糖（WPS）的衍生物，以及内毒素的水解裂解产物（包括脂多糖A和无毒多糖（PS）），就其在体外诱导小鼠脾细胞有丝分裂增殖的能力以及增强对绵羊红细胞抗体形成的能力进行了研究。完整的内毒素和脂多糖A成分诱导正常淋巴细胞产生显著的增殖活性，但内毒素经30分钟或更长时间温和水解后得到的PS成分有丝分裂活性要低得多。WPS制剂在30分钟后仍保留有丝分裂活性，但在持续水解过程中丧失了该活性。然而，就增强对绵羊红细胞的抗体反应而言，所有这些成分在体外对小鼠脾细胞都是显著的佐剂。完整的内毒素以及脂多糖A成分在体外刺激脾细胞培养24小时后均诱导产生白细胞介素-1和干扰素，包括α/β和γ干扰素。虽然只有内毒素和脂多糖A而不是PS成分对小鼠脾细胞有丝分裂原性，但所有这些制剂都有免疫佐剂作用，并诱导脾细胞产生或释放干扰素。然而，富含PS的衍生物不诱导肿瘤坏死因子。内毒素各成分的这些不同特性表明，与有毒的内毒素或脂多糖A相比，无毒的富含PS的衍生物具有一些免疫刺激活性，但缺乏其他一些活性。
