Rothman J, Johnson A G, Friedman H, Kovats E, Pham P H, Sanavi F, Nowotny A M, Nowotny A
J Biol Response Mod. 1985 Apr;4(2):169-84.
The White-type polysaccharide (WPS), often called Freeman polysaccharide, was obtained by hydrolyzing gram-negative bacteria in 0.2 N acetic acid at 100 degrees C for 2 h. The crude product contained partially degraded O-antigens as well as other components that were active as immune adjuvants, enhancers of macrophage cytotoxicity of tumor target cells, and inducers of osteoclastic bone resorption. The same WPS preparation augmented the tumor cytotoxicity of normal mouse spleen cells and slightly retarded the take of L1210 leukemia in mice. The WPS preparations generated colony-stimulating factor in mice but were not active in lymphoproliferative (mitogenicity) tests. Chemical analyses of the WPS preparations did not detect the presence of components characteristic of the lipid moiety of endotoxins. The WPS samples were also negative in biological assays of endotoxicity such as local Shwartzman and toxicity tests. These findings indicate that gram-negative bacteria contain nonendotoxin components that are potent modifiers of some biological responses.
白型多糖（WPS），通常称为弗里曼多糖，是通过在100℃下于0.2N乙酸中水解革兰氏阴性菌2小时而获得的。粗产物含有部分降解的O抗原以及其他作为免疫佐剂、肿瘤靶细胞巨噬细胞细胞毒性增强剂和破骨细胞骨吸收诱导剂具有活性的成分。相同的WPS制剂增强了正常小鼠脾细胞的肿瘤细胞毒性，并略微延缓了小鼠L1210白血病的发生。WPS制剂在小鼠中产生集落刺激因子，但在淋巴细胞增殖（促有丝分裂）试验中无活性。对WPS制剂的化学分析未检测到内毒素脂质部分特征成分的存在。WPS样品在内毒素生物学测定如局部施瓦茨曼反应和毒性试验中也呈阴性。这些发现表明革兰氏阴性菌含有非内毒素成分，这些成分是某些生物学反应的有效调节剂。
