Borden P, Kabat E A
Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Mol Immunol. 1988 Mar;25(3):251-62. doi: 10.1016/0161-5890(88)90016-8.
The specificities of polyclonal and monoclonal anti-idiotypes to three anti-alpha(1----6)dextrans-10.16.1, QUPC52, and W3129--were examined by competition ELISA. A major idiotype was defined by two polyclonal and two monoclonal anti-idiotypes to 10.16.1, and a polyclonal anti-idiotype to QUPC52. Another monoclonal anti-idiotype to 10.16.1 defines a non-overlapping determinant. One monoclonal anti-idiotype to 10.16.1 and one to W3129 were hapten inhibitable. By comparing amino acid sequences of Id+ and Id- anti-alpha(1----6)dextrans, the major idiotype was assigned to residues in VH CDR3, with a possible contribution from VH CDR2, a conclusion supported by the hapten inhibition results. Both a monoclonal and a previously described polyclonal anti-idiotype to W3129 define a determinant found on only W3129, among the anti-alpha(1----6)dextrans tested.
通过竞争ELISA检测了多克隆和单克隆抗独特型针对三种抗α(1→6)葡聚糖（10.16.1、QUPC52和W3129）的特异性。两种针对10.16.1的多克隆抗独特型、两种针对10.16.1的单克隆抗独特型以及一种针对QUPC52的多克隆抗独特型确定了一种主要独特型。另一种针对10.16.1的单克隆抗独特型定义了一个不重叠的决定簇。一种针对10.16.1的单克隆抗独特型和一种针对W3129的单克隆抗独特型可被半抗原抑制。通过比较Id+和Id-抗α(1→6)葡聚糖的氨基酸序列，主要独特型被定位到VH CDR3中的残基，VH CDR2可能也有贡献，半抗原抑制结果支持了这一结论。在检测的抗α(1→6)葡聚糖中，一种针对W3129的单克隆抗独特型和先前描述的一种针对W3129的多克隆抗独特型都定义了仅在W3129上发现的一个决定簇。
